University of Oulu

Some applications of RF-gradients and excitation sculpting in NMR spectroscopy

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Author: Heikkinen, Sami1
Organizations: 1University of Oulu, Faculty of Science, Department of Chemistry
Format: ebook
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.3 MB)
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Language: English
Published: 1999
Publish Date: 1999-05-11
Thesis type: Doctoral Dissertation
Defence Note: Academic Dissertation to be presented with the assent of the Faculty of Science, University of Oulu, for public discussion in Raahensali (Auditorium L 10), Linnanmaa, on May 22nd, 1999, at 12 noon.
Reviewer: Professor Jukka Jokisaari
Professor Jorma Mattinen


RF-gradients produced utilizing RF-field inhomogenity of conventional receiver/transmitter coil of NMR-probe can be used to mimic the effects of B0-gradients. This is done by placing long inhomogenous pulse in between two 90° pulses of appropriate phases (z-rotation cluster). B0-gradient based excitation sculpting can be converted into RF-gradient version. Selective one-dimensional TOCSY and NOESY using RF-gradient based excitation sculpting are described. In addition, non-selective two-dimensional experiments, TOCSY and NOESY, with RF-gradient based coherence selection are presented. Excitation sculpting using BIRD or BIRDR as inversion element results in isotope filter. Pre-suppression of non-13C-bound protons using RF-gradient BIRD prior to HSQC enables recording of spectrum of comparable quality to B0-gradient selected HSQC. This is beneficial for spectrometers lacking B0-gradient capabilities. Excitation sculpting using BIRDR can be used efficiently as low-pass filter in HMBC experiment. Furthermore, simultaneous elimination of protons bound to 15N and 13C can be accomplished with BIRDR based method.

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Series: Acta Universitatis Ouluensis. A, Scientiae rerum naturalium
ISSN-E: 1796-220X
ISBN: 951-42-5235-7
ISBN Print: 951-42-5233-0
Issue: 326
Copyright information: © University of Oulu, 1999. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited.