University of Oulu

Sucrose load, calcium-deficiency and dental caries on molars of growing rats

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Author: Pekkala, Esa1,2
Organizations: 1University of Oulu, Department of Pedodontics, Cariology and Endodontics
2University of Oulu, Faculty of Medicine, Institute of Dentistry, Department of Oral and Maxillofacial Surgery
Format: ebook
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.8 MB)
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Language: English
Published: 2003
Publish Date: 2003-06-14
Thesis type: Doctoral Dissertation
Defence Note: Academic Dissertation to be presented with the assent of the Faculty of Medicine, University of Oulu, for public discussion in the Auditorium 1 of the Institute of Dentistry, on June 14th, 2003, at 12 noon.
Reviewer: Professor John Leonora
Docent Juha Tuukkanen


The effects of dietary sucrose feeding, intragastric sucrose feeding and dietary calcium-deficiency on primary dentinogenesis of the pulp-dentin organ and caries progression were examined in an experimental rat model. The possible role of calcium balance and reduced mineralization of dentin organic matrix as a cause of reduced dentin formation in young, fast growing, rats were also studied. During 3-6 weeks of feeding, immediately after weaning at 3 weeks the urinary calcium, phosphorus, potassium and sodium levels and excretion rates were determined. The areas of dentin formed, the width of the predentin layer, serum mineral and insulin levels, and the areas of dentinal caries lesions were quantified at the end of the experiment. Also the occurrence and progression level of caries lesions were measured. In rat pups, dietary sucrose reduced dentin formation both during the lactation and experimental periods, increased urinary Ca2+ excretions, reduced urinary P, K and Na excretions, and enhanced dental caries occurrence and progression, but it did not affect the width of the predentin layer or the serum mineral and insulin levels. Intragastric sucrose reduced dentin formation and increased Ca2+ excretion, but did not affect the width of the predentin layer, serum mineral and insulin levels in the blood or induce dental caries. Dietary calcium-deficiency reduced dentin formation, increased the width of the predentin layer, caused hypocalcemia, and reduced urinary Ca2+ excretion. These results show that sucrose and calcium-deficiency reduce the rate of primary dentinogenesis through different mechanisms. Calcium imbalance or reduced mineralization of the dentin organic matrix does not explain the reduced dentinogenesis in sucrose fed rats. In conclusion, the present findings indicate that a sucrose load reduces dentinogenesis by impairing the synthesis rate of the dentin organic matrix, but also points out the importance of the local sucrose challenge in initiating dental caries.

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Series: Acta Universitatis Ouluensis. D, Medica
ISSN-E: 1796-2234
ISBN: 951-42-7035-5
ISBN Print: 951-42-7034-7
Issue: 729
Copyright information: © University of Oulu, 2003. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited.