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Plasmon-resonant gold nanoparticles for bioimaging and sensing applications |
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| Author: | Bibikova, Olga1,2,3,4,5,6,7 |
| Organizations: |
1University of Oulu Graduate School 2University of Oulu, Faculty of Information Technology and Electrical Engineering, Electrical Engineering 3University of Oulu, Infotech Oulu
4University of Oulu, Faculty of Biochemistry and Molecular Medicine
5University of Oulu, Biocenter Oulu 6International Graduate School in Molecular Medicine 7Institute of Analytical and Bioanalytical Chemistry, Ulm University, Germany |
| Format: | ebook |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 8.4 MB) |
| Persistent link: | http://urn.fi/urn:isbn:9789526219974 |
| Language: | English |
| Published: |
Oulu : University of Oulu,
2018
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| Publish Date: | 2018-09-04 |
| Thesis type: | Doctoral Dissertation |
| Defence Note: | Academic dissertation to be presented with the assent of the Doctoral Training Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the OP auditorium (L10), Linnanmaa, on 14 September 2018, at 12 noon |
| Tutor: |
Docent Matti Kinnunen Docent Alexey Popov Professor Krisztian Kordas Doctor Ilya Skovorodkin Professor Boris Mizaikoff |
| Reviewer: |
Professor Ricardas Rotomskis Doctor Vladimir Sivakov |
| Opponent: |
Professor Walter Blondel Doctor Vladimir Sivakov |
| Description: |
AbstractThis thesis reports on studies of plasmonic nanoparticles and particularly gold nanostars as signal enhancers and contrast agents for biophotonic applications including visualisation, treatment of living cells and chemical sensing. In this thesis, the optical properties of nanoparticles of different size and morphology and their silica composites were compared. Because they are the most suitable plasmonic nanostructures, gold nanostars were utilised for optical imaging modalities such as confocal microscopy and Doppler optical coherence tomography. The ability of gold nanoparticles to enhance the signal in surface-enhanced vibrational spectroscopy, including Raman and Fourier transform infrared spectroscopy was additionally studied. Finally, various gold nanoparticles were applied for cell optoporation to increase the penetration ability of exogeneous substances. In summary, significant advantages of nanostars such as their low-toxicity, high scattering and contrast abilities, in addition to a broad, tunable, plasmon resonance wavelength range, as well as the capability to enhance the signal of analyte molecules in vibrational spectroscopy were demonstrated in this thesis. The results of this study on the effectiveness of nanostars have a broad scope of utility and open a wide perspective for their utilisation in nanobiophotonics and biomedicine. see all
TiivistelmäTämä opinnäytetyö kertoo tutkimuksista, joissa plasmoninanopartikkeleita ja erityisesti kultananotähtiä on käytetty signaalinvahvistimina biofotoniikan sovelluksissa, kuten visualisointi, elävien solujen käsittely ja kemiallinen tunnistus. Tässä työssä verrattiin eri kokoisten ja muotoisten nanopartikkeleiden ja niiden piioksidikomposiittien optisia ominaisuuksia. Sopivimpina plasmoninanorakenteina kultananotähtiä käytettiin optisiin kuvantamismenetelmiin, kuten konfokaalimikroskopiaan ja Doppler-optiseen koherenssitomografiaan. Lisäksi kuvattiin myös kultananopartikkelien kykyä parantaa pinta-aktivoidun värähtelevän spektroskopian signaalia, mukaan lukien Raman- ja Fourier-muunnos-infrapuna-spektroskopia. Lopuksi, eri kultananopartikkeleita käytettiin soluoptoporaatioon eksogeenisten aineiden läpäisevyyden lisäämiseksi. Yhteenvetona, työssä osoitettiin nanotähtien merkittävät edut, kuten matala-myrkyllisyys, suuret sironta- ja kontrastiominaisuudet, laaja plasmoniresonanssin aallonpituusalue ja sen viritettävyys, sekä kyky parantaa analyyttimolekyylien signaalia värähtelyspektroskopiassa. Niinpä tutkimustulokset nanotähtien tehokkuudesta ovat laajasti käyttökelpoisia ja ne avaavat laajan näkökulman niiden hyödyntämiseen nanobiofotoniikassa ja biolääketieteessä. see all
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| Series: |
Acta Universitatis Ouluensis. C, Technica |
| ISSN: | 0355-3213 |
| ISSN-E: | 1796-2226 |
| ISSN-L: | 0355-3213 |
| ISBN: | 978-952-62-1997-4 |
| ISBN Print: | 978-952-62-1996-7 |
| Issue: | 672 |
| Subjects: | |
| Copyright information: |
© University of Oulu, 2018. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited. |