University of Oulu

Interaction peculiarities of red blood cells and hemorheological alterations induced by laser radiation

Saved in:
Author: Zhu, Ruixue1,2
Organizations: 1University of Oulu Graduate School
2University of Oulu, Faculty of Information Technology and Electrical Engineering, Electrical Engineering, Opto-Electronics and Measurement Technicues (OPEM)
Format: ebook
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4 MB)
Persistent link: http://urn.fi/urn:isbn:9789526233543
Language: English
Published: Oulu : University of Oulu, 2022
Publish Date: 2022-06-23
Thesis type: Doctoral Dissertation
Defence Note: Academic dissertation to be presented with the assent of the Doctoral Programme Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the OP auditorium (L10), Linnanmaa, on 30 June 2022, at 12 noon
Tutor: Professor Alexey Popov
Professor Igor Meglinski
Reviewer: Professor Christian Wagner
Professor Honglian Guo
Opponent: Professor Shangran Xie
Professor Christian Wagner
Description:

Abstract

This thesis reports on studies of the fundamental interaction dynamics of human red blood cells (RBC) by optical tweezers (OT) and the application of the OT-based RBC-investigation protocol in facilitating blood photobiomodulation research at a single-cell level. The motivation for the present study arises from the scientific and clinical significance of examining RBC interaction mechanisms as a model for studying general cell interaction in cytological science. Exploring the phenomenon and mechanism of the photobiomodulation of laser radiation on the rheological properties of RBC has a great potential in the field of laser blood therapy.

Interaction dynamics and the role of intercellular interaction time and mutual contact in RBC aggregation and OT-induced disaggregation in autologous plasma are presented as new evidence, clarifying the RBC interaction mechanism. The rheological alterations of RBC induced by laser radiation with various irradiation conditions were thoroughly explored at a single-cell level for the first time to provide a better understanding of the underlying mechanism of photobiomodulation on blood. This study demonstrated the beneficial effects of low-level laser irradiation by a 450-nm wavelength with a radiant exposure below 9.5 J/cm2 of improving RBC deformability and preserving cell shape in a harsh environment. Additionally, irradiated RBC aggregates were easily destroyed by the external influence (i.e., optical force), which could be the flow shearing force in a blood vessel and geometrical resistance by vasculature for in vivo conditions.

The current work is important for optimizing the technique of the OT-based RBC evaluation system. It will contribute to the development of effective approaches for improving blood cell viability and blood microcirculation based on the reported photobiomodulation effects.

see all

Tiivistelmä

Tässä opinnäytetyössä raportoidaan ihmisen punasolujen (RBC) perustavanlaatuisen vuorovaikutusdynamiikan tutkimuksista optisilla pinseteillä (OT) ja OT-pohjaisen RBC-tutkimusprotokollan soveltamisesta veren fotobiomodulaatiotutkimuksen helpottamiseksi yksisolutasolla. Motivaatio nykyiselle tutkimukselle syntyy punasolujen vuorovaikutusmekanismien tutkimisen tieteellisestä ja kliinisestä merkityksestä mallina yleisen soluvuorovaikutuksen tutkimiseen sytologiassa. Kun lasersäteilyn fotobiomodulaation ilmiötä ja mekanismia tutkitaan punasolujen reologisissa ominaisuuksissa, siinä on paljon potentiaalia laserveriterapian alalla.

Interaktiodynamiikka ja solunvälisen interaktioajan ja keskinäisen kontaktin rooli punasolujen aggregaatiossa ja OT:n aiheuttamassa hajoamisessa autologisessa plasmassa esitetään uutena todisteena punasolujen yhteisvaikutusmekanismia selventävänä näyttönä. Punasoluissa tapahtuneita, lasersäteilyn erilaisissa valaistusolosuhteissa aiheuttamia reologisia muutoksia tarkasteltiin nyt ensimmäistä kertaa perusteellisesti yksittäisten solujen tasolla, minkä seurauksena saatiin aiempaa parempi ymmärrys veren fotobiomodulaation taustalla olevasta mekanismista. Tutkimus havainnollisti matalaenergisen, teholtaan alle 9,5 J/cm2:n säteilyaltistuksen ja aallonpituudeltaan 450 nm:n lasersäteilyn hyödylliset vaikutukset punasolujen muodonmuutoksen parantamisessa ja solujen kyvyssä säilyttää muotonsa ankarissakin olosuhteissa. Lisäksi säteilytetyt RBC-aggregaatit tuhoutuivat helposti ulkoisen vaikutuksen vaikutuksesta (optinen voima), joka voi olla virtauksen leikkausvoima verisuonessa ja geometrinen vastus verisuonissa in vivo -olosuhteissa.

Tutkimuksen saavutukset ovat merkittäviä optimoitaessa optiseen pinsettiin perustuvan punasolujen arviointijärjestelmän tekniikoita. Raportoitujen fotobiomodulaatio vaikutusten kautta työ edistää verisolujen elinkelpoisuuden ja veren mikrovirtauksen parantamiseen tähtäävien tehokkaiden lähestymistapojen kehittämistä.

see all

Osajulkaisut / Original papers

Osajulkaisut eivät sisälly väitöskirjan elektroniseen versioon. / Original papers are not included in the electronic version of the dissertation.

  1. Zhu, R., Avsievich, T., Su, X., Bykov, A., Popov, A., & Meglinski, I. (2022). Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation. Journal of Photochemistry and Photobiology B: Biology, 230, 112438. https://doi.org/10.1016/j.jphotobiol.2022.112438

  2. Zhu, R., Avsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2021). In vivo nano-biosensing element of red blood cell-mediated delivery. Biosensors and Bioelectronics, 175, 112845. https://doi.org/10.1016/j.bios.2020.112845

    Rinnakkaistallennettu versio / Self-archived version

  3. Zhu, R., Avsievich, T., Bykov, A., Popov, A., & Meglinski, I. (2019). Influence of pulsed He–Ne laser irradiation on the red blood cell interaction studied by optical tweezers. Micromachines, 10(12), 853. https://doi.org/10.3390/mi10120853

    Rinnakkaistallennettu versio / Self-archived version

  4. Zhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2020). Optical tweezers in studies of red blood cells. Cells, 9(3), 545. https://doi.org/10.3390/cells9030545

    Rinnakkaistallennettu versio / Self-archived version

  5. Zhu, R., Avsievich, T. I., Popov, A., Bykov, A., & Meglinski, I. (2021). In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. Proceedings of SPIE, Optical Trapping and Optical Micromanipulation XVIII 11798, 1179809. https://doi.org/10.1117/12.2595118

    Rinnakkaistallennettu versio / Self-archived version

  6. Zhu, R., Popov, A., & Meglinski, I. (2020). Probing the red blood cell interaction in individual cell pairs by optical tweezers. Conference on Lasers and Electro-optics, AW3I.4. https://doi.org/10.1364/CLEO_AT.2020.AW3I.4

  7. Zhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2019). Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers. Proceedings of SPIE, Novel Biophotonics Techniques and Applications V 11075, 110750D. https://doi.org/10.1117/12.2526778

    Rinnakkaistallennettu versio / Self-archived version

see all

Series: Acta Universitatis Ouluensis. C, Technica
ISSN: 0355-3213
ISSN-E: 1796-2226
ISSN-L: 0355-3213
ISBN: 978-952-62-3354-3
ISBN Print: 978-952-62-3353-6
Issue: 834
Type of Publication: G5 Doctoral dissertation (articles)
Field of Science: 213 Electronic, automation and communications engineering, electronics
Subjects:
Funding: I would like to acknowledge the financial support provided by the China Scholarship Council (CSC No. 201706410089), STSM Grant from COST Action CA17140 “Cancer Nanomedicine from the Bench to the Bedside” supported by COST (European Cooperation in Science and Technology, grant No. ECOST-STSM-CA17140-230,919-113049), Tauno Tönning Foundation (grant No. 20190104), and the University of Oulu Graduate School (UniOGS) travel grants.
Copyright information: © University of Oulu, 2022. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited.