University of Oulu

Lappalainen OP, Karhula SS, Haapea M, Kauppinen S, Finnilä M, Saarakkala S, Serlo W, Sándor GK. Micro-CT Analysis of Bone Healing in Rabbit Calvarial Critical-Sized Defects with Solid Bioactive Glass, Tricalcium Phosphate Granules or Autogenous Bone J Oral Maxillofac Res 2016;7(2):e4 URL: doi: 10.5037/jomr.2016.7204

Micro-CT analysis of bone healing in rabbit calvarial critical-sized defects with solid bioactive glass, tricalcium phosphate granules or autogenous bone

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Author: Lappalainen, Olli-Pekka1; Karhula, Sakari S.2,3; Haapea, Marianne3;
Organizations: 1Department of Oral and Maxillofacial Surgery, Research Group in Tissue Engineering, Faculty of Medicine, Medical Research Center, University of Oulu, Oulu University Hospital, Oulu, Finland
2Department of Medical Imaging, Physics and Technology, Research Unit, Faculty of Medicine, Medical Research Center, University of Oulu, Oulu, Finland
3Department of Diagnostic Radiology, Oulu University Hospital, Medical Research Center, University of Oulu, Oulu, Finland
4Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
5Department of Pediatric Surgery, PEDEGO Research Center, Oulu University Hospital, Medical Research Center, Uinversity of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
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Language: English
Published: Stilus Optimus, 2016
Publish Date: 2017-02-01


Objectives: The purpose of the present study was to evaluate bone healing in rabbit critical-sized calvarial defects using two different synthetic scaffold materials, solid biodegradable bioactive glass and tricalcium phosphate granules alongside solid and particulated autogenous bone grafts.

Material and Methods: Bilateral full thickness critical-sized calvarial defects were created in 15 New Zealand white adult male rabbits. Ten defects were filled with solid scaffolds made of bioactive glass or with porous tricalcium phosphate granules. The healing of the biomaterial-filled defects was compared at the 6 week time point to the healing of autologous bone grafted defects filled with a solid cranial bone block in 5 defects and with particulated bone combined with fibrin glue in 10 defects. In 5 animals one defect was left unfilled as a negative control. Micro-computed tomography (micro-CT) was used to analyze healing of the defects.

Results: Micro-CT analysis revealed that defects filled with tricalcium phosphate granules showed new bone formation in the order of 3.89 (SD 1.17)% whereas defects treated with solid bioactive glass scaffolds showed 0.21 (SD 0.16)%, new bone formation. In the empty negative control defects there was an average new bone formation of 21.8 (SD 23.7)%.

Conclusions: According to findings in this study, tricalcium phosphate granules have osteogenic potential superior to bioactive glass, though both particulated bone with fibrin glue and solid bone block were superior defect filling materials.

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Series: Journal of oral & maxillofacial research
ISSN: 2029-283X
ISSN-E: 2029-283X
ISSN-L: 2029-283X
Volume: 7
Issue: 2
Article number: e4
DOI: 10.5037/jomr.2016.7204
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
Funding: The authors wish to express their gratitude for the financial support provided to this project by the ITI Foundation, Basel, Switzerland (ITI Grant number 619-2009), the University of Oulu EVO and VTR Grant Fund, the Stiftelsen Alma och K.A. Snellman Foundation, Oulu, Finland, Academy of Finland (Grants no. 268378 and 273571), The Sigrid Juselius Foundation and European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 336267, for their generosity.
Academy of Finland Grant Number: 268378
Detailed Information: 268378 (Academy of Finland Funding decision)
273571 (Academy of Finland Funding decision)
Copyright information: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2016.