University of Oulu

Ivana Zagotta, Elitsa Y. Dimova, Jan-Bernd Funcke, Martin Wabitsch, Thomas Kietzmann, and Pamela Fischer-Posovszky, “Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue,” Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 793525, 13 pages, 2013. doi:10.1155/2013/793525

Resveratrol suppresses PAI-1 gene expression in a human in vitro model of inflamed adipose tissue

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Author: Zagotta, Ivana1; Dimova, Elitsa Y.2; Funcke, Jan-Bernd1;
Organizations: 1Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Eythstraße 24, 89075 Ulm, Germany
2Department of Biochemistry and Biocenter Oulu, University of Oulu, P.O.B. 3000, 90014 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.1 MB)
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Language: English
Published: Hindawi, 2013
Publish Date: 2017-03-15


Increased plasminogen activator inhibitor-1 (PAI-1) levels are associated with a number of pathophysiological complications; among them is obesity. Resveratrol was proposed to improve obesity-related health problems, but the effect of resveratrol on PAI-1 gene expression in obesity is not completely understood. In this study, we used SGBS adipocytes and a model of human adipose tissue inflammation to examine the effects of resveratrol on the production of PAI-1. Treatment of SGBS adipocytes with resveratrol reduced PAI-1 mRNA and protein in a time- and concentration-dependent manner. Further experiments showed that obesity-associated inflammatory conditions lead to the upregulation of PAI-1 gene expression which was antagonized by resveratrol. Although signaling via PI3K, Sirt1, AMPK, ROS, and Nrf2 appeared to play a significant role in the modulation of PAI-1 gene expression under noninflammatory conditions, those signaling components were not involved in mediating the resveratrol effects on PAI-1 production under inflammatory conditions. Instead, we demonstrate that the resveratrol effects on PAI-1 induction under inflammatory conditions were mediated via inhibition of the NFκB pathway. Together, resveratrol can act as NFκB inhibitor in adipocytes and thus the subsequently reduced PAI-1 expression in inflamed adipose tissue might provide a new insight towards novel treatment options of obesity.

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Series: Oxidative medicine and cellular longevity
ISSN: 1942-0900
ISSN-E: 1942-0994
ISSN-L: 1942-0900
Volume: 2013
Article number: 793525
DOI: 10.1155/2013/793525
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: PFP was funded by aMargarete vonWrangell scholarship financed by the Baden-Wuerttemberg Ministry of Science, Research and Arts; the European Social Fund; and Ulm University. Ivana Zagotta is funded by the International Graduate School in Molecular Medicine Ulm. This study was in part supported by the Research Training Group GRK 1041 “Molecular Diabetology and Endocrinology inMedicine” to Pamela Fischer- Posovszky and Martin Wabitsch and the foundation “Das zuckerkranke Kind” to Pamela Fischer-Posovszky. Work in the Thomas Kietzmann lab is supported by grants from the Biocenter Oulu, Academy of Finland, and Sigrid Juselius Foundation.
Copyright information: Copyright © 2013 Ivana Zagotta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.