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A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity |
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| Author: | Shimomoto , Takasumi1; Collins , Leonard B.1; Yi, Xianwen2; |
| Organizations: |
1Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina, United States of America 2Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, United States of America 3Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, United States of America
4Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina, United States of America
5School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan 6Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland 7Medical Research Center and Nordlab Oulu, University Hospital and University of Oulu, Oulu, Finland 8Department of Pathology & Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, United States of America 9McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina, United States of America |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 1.1 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe201704256251 |
| Language: | English |
| Published: |
Public Library of Science,
2017
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| Publish Date: | 2017-04-25 |
| Description: |
AbstractAtherosclerosis is widely accepted to be a chronic inflammatory disease, and the immunological response to the accumulation of LDL is believed to play a critical role in the development of this disease. 1,4-Dihydropyridine-type MAA-adducted LDL has been implicated in atherosclerosis. Here, we have demonstrated that pure MAA-modified residues can be chemically conjugated to large proteins without by-product contamination. Using this pure antigen, we established a purified MAA-ELISA, with which a marked increase in anti-MAA antibody titer was determined at a very early stage of atherosclerosis in 3-month ApoE-/- mice fed with a normal diet. Our methods of Nε-MAA-L-lysine purification and purified antigen-based ELISA will be easily applicable for biomarker-based detection of early stage atherosclerosis in patients, as well as for the development of an adduct-specific Liquid Chromatography/Mass Spectrometry-based quantification of physiological and pathological levels of MAA. see all
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| Series: |
PLoS one |
| ISSN: | 1932-6203 |
| ISSN-E: | 1932-6203 |
| ISSN-L: | 1932-6203 |
| Volume: | 12 |
| Issue: | 2 |
| Article number: | e72172 |
| DOI: | 10.1371/journal.pone.0172172 |
| OADOI: | https://oadoi.org/10.1371/journal.pone.0172172 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
3121 General medicine, internal medicine and other clinical medicine |
| Subjects: | |
| Funding: |
This work was supported in part by NIH grants P42-ES05948 and P30-ES10126. |
| Copyright information: |
© 2017 Shimomoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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https://creativecommons.org/licenses/by/4.0/ |