University of Oulu

Shimomoto T, Collins LB, Yi X, Holley DW, Zhang Z, Tian X, et al. (2017) A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity. PLoS ONE 12(2): e0172172. https://doi.org/10.1371/journal.pone.0172172

A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity

Saved in:
Author: Shimomoto , Takasumi1; Collins , Leonard B.1; Yi, Xianwen2;
Organizations: 1Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina, United States of America
2Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, United States of America
3Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, United States of America
4Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina, United States of America
5School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
6Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland
7Medical Research Center and Nordlab Oulu, University Hospital and University of Oulu, Oulu, Finland
8Department of Pathology & Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, United States of America
9McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina, United States of America
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201704256251
Language: English
Published: Public Library of Science, 2017
Publish Date: 2017-04-25
Description:

Abstract

Atherosclerosis is widely accepted to be a chronic inflammatory disease, and the immunological response to the accumulation of LDL is believed to play a critical role in the development of this disease. 1,4-Dihydropyridine-type MAA-adducted LDL has been implicated in atherosclerosis. Here, we have demonstrated that pure MAA-modified residues can be chemically conjugated to large proteins without by-product contamination. Using this pure antigen, we established a purified MAA-ELISA, with which a marked increase in anti-MAA antibody titer was determined at a very early stage of atherosclerosis in 3-month ApoE-/- mice fed with a normal diet. Our methods of Nε-MAA-L-lysine purification and purified antigen-based ELISA will be easily applicable for biomarker-based detection of early stage atherosclerosis in patients, as well as for the development of an adduct-specific Liquid Chromatography/Mass Spectrometry-based quantification of physiological and pathological levels of MAA.

see all

Volume: 12
Issue: 2
Article number: e72172
DOI: 10.1371/journal.pone.0172172
OADOI: https://oadoi.org/10.1371/journal.pone.0172172
Type of Publication: A1 Journal article – refereed
Field of Science: 3121 Internal medicine
Subjects:
Funding: This work was supported in part by NIH grants P42-ES05948 and P30-ES10126.
Copyright information: © 2017 Shimomoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  https://creativecommons.org/licenses/by/4.0/