University of Oulu

Moilanen, J. M. et al. Significant Role of Collagen XVII And Integrin β4 In Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells. Sci. Rep. 7, 45057; doi: 10.1038/srep45057 (2017).

Significant role of collagen XVII and integrin β4 in migration and invasion of the less aggressive squamous cell carcinoma cells

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Author: Moilanen, Jyri M.1; Löffek, Stefanie2; Kokkonen, Nina1;
Organizations: 1Department of Dermatology, PEDEGO Research Unit, Oulu Center for Cell-Matrix Research, MRC Oulu, University of Oulu and Oulu University Hospital, Finland
2Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University of Duisburg-Essen, Germany
3Oulu Center for Cell-Matrix Research, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
4Department of Pathology, Research Unit of Cancer and Translational Medicine, MRC Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland
5Department of Dermatology, Turku University Hospital, MediCity Research Laboratory, University of Turki, Turku, Finland
6Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany
7Research Unit of Cancer and Translational Medicine, MRC Oulu, University of Oulu and Oulu University Hospital, Finland
8Department of Oral and Maxillo-facial Diseases, University of Helsinki, Finland
9HUSLAB, Department of Pathology, Helsinki University Central Hospital, Finland
10Department of Oral Diagnosis, Oral Pathology Division, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, SP-13414-903, Brazil
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.9 MB)
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Language: English
Published: Springer Nature, 2017
Publish Date: 2017-05-18


Collagen XVII and integrin α6β4 have well-established roles as epithelial adhesion molecules. Their binding partner laminin 332 as well as integrin α6β4 are largely recognized to promote invasion and metastasis in various cancers, and collagen XVII is essential for the survival of colon and lung cancer stem cells. We have studied the expression of laminin γ2, collagen XVII and integrin β4 in tissue microarray samples of squamous cell carcinoma (SCC) and its precursors, actinic keratosis and Bowen’s disease. The expression of laminin γ2 was highest in SCC samples, whereas the expression of collagen XVII and integrin β4 varied greatly in SCC and its precursors. Collagen XVII and integrin β4 were also expressed in SCC cell lines. Virus-mediated RNAi knockdown of collagen XVII and integrin β4 reduced the migration of less aggressive SCC-25 cells in horizontal scratch wound healing assay. Additionally, in a 3D organotypic myoma invasion assay the loss of collagen XVII or integrin β4 suppressed equally the migration and invasion of SCC-25 cells whereas there was no effect on the most aggressive HSC-3 cells. Variable expression patterns and results in migration and invasion assays suggest that collagen XVII and integrin β4 contribute to SCC tumorigenesis.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 7
Article number: 45057
DOI: 10.1038/srep45057
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
3122 Cancers
Funding: This study was supported by the Academy of Finland, Oulu University Hospital and Northern Finland Cancer Association grants to N.K. and K.T. The work of P.R. and V.-M.K. was supported by Turku University Hospital (project 13336), Sigrid Jusélius Foundation and Cancer Research Foundation of Finland.
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