Huhta, Heikki; Helminen, Olli; Palomäki, Sami; Kauppila, Joonas H.; Saarnio, Juha; Lehenkari, Petri P.; Karttunen, Tuomo J. Intratumoral lactate metabolism in Barrett’s esophagus and adenocarcinoma. Oncotarget. 2017; 8:22894-22902. doi: 10.18632/oncotarget.15284
Intratumoral lactate metabolism in Barrett’s esophagus and adenocarcinoma
|Author:||Huhta, Heikki1,2,3,4; Helminen, Olli1,2,3,4; Palomäki, Sami3,4;|
1Departments of Pathology, University of Oulu, Oulu, Finland
2Departments of Surgery, University of Oulu, Oulu, Finland
3Medical Research Center Oulu, Oulu, Finland
4Oulu University Hospital, Oulu, Finland
5Upper Gastrointestinal Surgery, Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
|Online Access:||PDF Full Text (PDF, 4.5 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe201705226767
|Publish Date:|| 2017-06-02
Background: Monocarboxylate transporters (MCTs) are cell membrane proteins which transport pyruvate, lactate and ketone bodies across the plasma membrane. MCTs are activated in various cancers, but their expression in esophageal adenocarcinoma is not known. The present study was conducted to elucidate the expression of MCTs in esophageal adenocarcinoma and its precursor lesions.
Results: Cytoplasmic MCT1, MCT4 and MTCO1 expression linearly increased from normal epithelium to Barrett’s mucosa to dysplasia and cancer. Low cytoplasmic MCT1 expression associated with high T-class (P < 0.01), positive lymph node metastases (P < 0.05), positive distant metastases (P < 0.01) and high tumor stage (P < 0.01). High cytoplasmic MCT4 expression correlated significantly with positive distant metastases (P < 0.05). Both low MCT1 and high MCT4 histoscore predicted survival in univariate analysis (P < 0.01). MCT4 histoscore predicted survival in multivariate analysis (P = 0.043; HR 1.8 95%CI 1.0–3.1). MTCO1 expression was not correlated to clinicopathological variables or survival.
Materials and Methods: MCT1, MCT4 and mitochondrial cytochrome c oxidase (MTCO1) expression were determined with immunohistochemistry in esophageal specimens from 129 patients with columnar dysplasia or adenocarcinoma. Specimens including normal esophagus (n = 88), gastric (n = 67) or intestinal metaplasia (n = 51), low-grade (n = 42), high-grade dysplasia (n = 37) and esophageal adenocarcinoma (n = 99) were evaluated.
Conclusions: Major increase in markers of tumor metabolism occurs during carcinogenesis and progression of esophageal adenocarcinoma. MCT1 and MCT4 are prognostic factors in esophageal adenocarcinoma.
|Pages:||22894 - 22902|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
3126 Surgery, anesthesiology, intensive care, radiology
This work was supported by grants from the Orion Research Foundation, Thelma Mäkikyrö Foundation, Päivikki and Sakari Sohlberg Foundation, the Emil Aaltonen Foundation, Georg C. and Mary Ehrnroot Foundation, Sigrid Jusélius Foundation, Finnish cancer foundation and the Finnish Medical Foundation.
© 2017 Huhta et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.