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Khatun M, Sorjamaa A, Kangasniemi M, Sutinen M, Salo T, Liakka A, et al. (2017) Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus. PLoS ONE 12(4): e0175986. https://doi.org/10.1371/journal.pone.0175986

Niche matters : the comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus

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Author: Khatun, Masuma1,2; Sorjamaa, Anna1,2; Kangasniemi, Marika1,2;
Organizations: 1Department of Obstetrics and Gynecology, PEDEGO Research Unit, Medical Research Center, University of Oulu, Oulu, Finland
2Oulu University Hospital, Oulu, Finland
3Cancer and Translational Medicine Research Unit, Medical Research Center, University of Oulu, Oulu, Finland
4Department of Pathology, Medical Research Center, University of Oulu, Oulu, Finland
5Department of Anatomy and Department of Internal Medicine, Medical Research Center, University of Oulu, Oulu, Finland
6Department of Obstetrics and Gynecology, University of Helsinki, Helsinki, Finland
7Helsinki University Central Hospital, Helsinki, Finland
8Research Unit of Biomedicine, University of Oulu, Oulu, Finland
9Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, United States of America
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201705296932
Language: English
Published: Public Library of Science, 2017
Publish Date: 2017-05-29
Description:

Abstract

Objective: Intrinsic inflammatory characteristics play a pivotal role in stem cell recruitment and homing through migration where the subsequent change in niche has been shown to alter these characteristics. The bone marrow mesenchymal stem cells (bmMSCs) have been demonstrated to migrate to the endometrium contributing to the stem cell reservoir and regeneration of endometrial tissue. Thus, the aim of the present study was to compare the inflammation-driven migration and cytokine secretion profile of human bmMSCs to endometrial mesenchymal stem cells (eMSCs) and endometrial fibroblasts (eSFs).

Materials and methods: The bmMSCs were isolated from bone marrow aspirates through culturing, whereas eMSCs and eSFs were FACS-isolated. All cell types were tested for their surface marker, proliferation profiles and migration properties towards serum and inflammatory attractants. The cytokine/chemokine secretion profile of 35 targets was analysed in each cell type at basal level along with lipopolysaccharide (LPS)-induced state.

Results: Both stem cell types, bmMSCs and eMSCs, presented with similar stem cell surface marker profiles as well as possessed high proliferation and migration potential compared to eSFs. In multiplex assays, the secretion of 16 cytokine targets was detected and LPS stimulation expanded the cytokine secretion pattern by triggering the secretion of several targets. The bmMSCs exhibited higher cytokine secretion of vascular endothelial growth factor (VEGF)-A, stromal cell-derived factor-1 alpha (SDF)-1α, interleukin-1 receptor antagonist (IL-1RA), IL-6, interferon-gamma inducible protein (IP)-10, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α and RANTES compared to eMSCs and/or eSFs after stimulation with LPS. The basal IL-8 secretion was higher in both endometrial cell types compared to bmMSCs.

Conclusion: Our results highlight that similar to bmMSCs, the eMSCs possess high migration activity while the differentiation process towards stromal fibroblasts seemed to result in loss of stem cell surface markers, minimal migration activity and a subtler cytokine profile likely contributing to normal endometrial function.

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Series: PLoS one
ISSN: 1932-6203
ISSN-E: 1932-6203
ISSN-L: 1932-6203
Volume: 12
Issue: 4
Article number: e0175986
DOI: 10.1371/journal.pone.0175986
OADOI: https://oadoi.org/10.1371/journal.pone.0175986
Type of Publication: A1 Journal article – refereed
Field of Science: 3123 Gynaecology and paediatrics
Subjects:
Article
Funding: This work was supported by Sigrid Juselius Foundation, Academy of Finland, Finnish Medical Foundation, and Orion-Farmos Research Foundation.
Dataset Reference: All relevant data are within the paper.
Copyright information: Copyright: ©2013 Jaakkonen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  https://creativecommons.org/licenses/by/1.0/