University of Oulu

Hirvonen et al. BMC Medical Genetics (2017) 18:41 DOI 10.1186/s12881-017-0401-z

SIRT6 polymorphism rs117385980 is associated with longevity and healthy aging in Finnish men

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Author: Hirvonen, Katariina1; Laivuori, Hannele2,3,4; Lahti, Jari5,6;
Organizations: 1The Folkhälsan Institute of Genetics and the Department of Medical Genetics, University of Helsinki
2Medical and Clinical Genetics and Obstetrics and Gynecology, University of Helsinki
3Helsinki University Hospital
4Institute for Molecular Medicine Finland, University of Helsinki
5Institute of Behavioural Sciences, University of Helsinki
6Folkhälsan Research Centre
7University of Helsinki
8Helsinki University Central Hospital, Geriatrics
9Institute of Health Sciences/Geriatrics, University of Oulu
10Department Chronic Disease Prevention, National Institute for Health and Welfare
11Department of General Practice and Primary Health Care, Helsinki University Hospital
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.3 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201706267467
Language: English
Published: BioMed Central, 2017
Publish Date: 2017-06-26
Description:

Abstract

Background Sirtuin-6 (SIRT6) is involved in various crucial cellular pathways, being a key regulator of telomere structure, DNA repair, metabolism, transcriptional control and the NF-kappa B pathway. Sirt6 knock-out mice have been reported to develop typical features of aging and senescence at the age of 2–3 weeks and die within 4 weeks. The aim of this study was to investigate whether sequence variations of SIRT6 are associated with aging and longevity in Finnish men.

Methods
The sample of this study consisted of 43 longer-living and healthy males and 92 male control subjects who have died of natural causes at an average age of 66,6 (±4,1) years and who belonged to the Helsinki Birth Cohort Study (HBCS). Single nucleotide polymorphisms (SNPs) in the exons and their surroundings of the SIRT6 were studied using direct PCR sequencing.

Results The SNP rs117385980 (C > T), situated 23 bases downstream of the exon 2 exon/intron border was found in heterozygous form in 1/43 longer-living healthy men (Minor allele frequency (MAF) 0,0116) and in 9/92 controls (MAF 0,0489). To replicate this finding, we studied a group of 63 healthy men at an average age of 83 years from the Helsinki Businessmen Study (HBS)–cohort. The heterozygosity of the same SNP was seen in 2/63 men from the HBS–cohort (MAF 0,0159). Fisher exact test was performed in our two combined study samples. The P-value for all samples combined was 0.07 and the odds ratio 3.53 (95% confidence interval 0.96–13.4).

Conclusion These results suggest an inverse association between the T allele of rs117385980 and longevity. The result needs to be confirmed in a larger study. It remains to be determined whether rs117385980 itself has an effect or if it is a mere genetic marker for some other yet undiscovered sequence variant causing a functional effect.
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Series: Bmc medical genetics
ISSN: 1471-2350
ISSN-E: 1471-2350
ISSN-L: 1471-2350
Volume: 18
Article number: 41
DOI: 10.1186/s12881-017-0401-z
OADOI: https://oadoi.org/10.1186/s12881-017-0401-z
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Funding: This study was supported by the Folkhälsan Research Foundation and the Magnus Ehrnrooths Foundation.
Dataset Reference: The dataset supporting the conclusions of this article is included within the article.
Copyright information: © The Author(s). 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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