University of Oulu

Annemarie Hempel, Susanne J. Kühl, Melanie Rothe, Purushothama Rao Tata, Ioan Ovidiu Sirbu, Seppo J. Vainio, Michael Kühl, The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis, Developmental Biology, Volume 424, Issue 1, 1 April 2017, Pages 28-39, ISSN 0012-1606, (

The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis

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Author: Hempel, Annemarie1,2; Kühl, Susanne J.1; Rothe, Melanie1,2,;
Organizations: 1Institute of Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, D-89081 Ulm, Germany
2International Graduate School in Molecular Medicine Ulm, IGradU, Ulm University, 89069 Ulm, Germany
3Current address: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27110, USA
4Current address: Victor Babes University of Medicine and Pharmacy Timisoara, Biochemistry Department, 2 Eftimie Murgu, 300041 Timisoara, Romania
5Faculty of Biochemistry and Molecular Medicine, BioCenter Oulu and InfoTech Oulu,University of Oulu, Aapistie 5, FIN-90014, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.9 MB)
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Language: English
Published: Elsevier, 2017
Publish Date: 2017-07-06


Wnt proteins are critical for embryonic cardiogenesis and cardiomyogenesis by regulating different intracellular signalling pathways. Whereas canonical Wnt/β-catenin signalling is required for mesoderm induction and proliferation of cardiac progenitor cells, β-catenin independent, non-canonical Wnt signalling regulates cardiac specification and terminal differentiation. Although the diverse cardiac malformations associated with the loss of non-canonical Wnt11 in mice such as outflow tract (OFT) defects, reduced ventricular trabeculation, myofibrillar disorganization and reduced cardiac marker gene expression are well described, the underlying molecular mechanisms are still not completely understood. Here we aimed to further characterize Wnt11 mediated signal transduction during vertebrate cardiogenesis. Using Xenopus as a model system, we show by loss of function and corresponding rescue experiments that the non-canonical Wnt signalling mediator Rcsd1 is required downstream of Wnt11 for ventricular trabeculation, terminal differentiation of cardiomyocytes and cardiac morphogenesis. We here place Rcsd1 downstream of Wnt11 during cardiac development thereby providing a novel mechanism for how non-canonical Wnt signalling regulates vertebrate cardiogenesis.

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Series: Developmental biology
ISSN: 0012-1606
ISSN-E: 1095-564X
ISSN-L: 0012-1606
Volume: 242
Issue: 1
Pages: 28 - 39
DOI: 10.1016/j.ydbio.2017.02.014
Type of Publication: A1 Journal article – refereed
Field of Science: 1184 Genetics, developmental biology, physiology
3111 Biomedicine
Funding: This work was supported by the Deutsche Forschungsgemeinschaft DFG (SI-1381/1-2 to IOS and Ku1166/3-2 to MK). AH, MR and PRT were supported by the International Graduate School in Molecular Medicine of Ulm University (GSC270).
Copyright information: © 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (