The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis |
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Author: | Hempel, Annemarie1,2; Kühl, Susanne J.1; Rothe, Melanie1,2,; |
Organizations: |
1Institute of Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, D-89081 Ulm, Germany 2International Graduate School in Molecular Medicine Ulm, IGradU, Ulm University, 89069 Ulm, Germany 3Current address: Department of Cell Biology, Duke University School of Medicine, Durham, NC 27110, USA
4Current address: Victor Babes University of Medicine and Pharmacy Timisoara, Biochemistry Department, 2 Eftimie Murgu, 300041 Timisoara, Romania
5Faculty of Biochemistry and Molecular Medicine, BioCenter Oulu and InfoTech Oulu,University of Oulu, Aapistie 5, FIN-90014, Finland |
Format: | article |
Version: | published version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.9 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe201707067658 |
Language: | English |
Published: |
Elsevier,
2017
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Publish Date: | 2017-07-06 |
Description: |
AbstractWnt proteins are critical for embryonic cardiogenesis and cardiomyogenesis by regulating different intracellular signalling pathways. Whereas canonical Wnt/β-catenin signalling is required for mesoderm induction and proliferation of cardiac progenitor cells, β-catenin independent, non-canonical Wnt signalling regulates cardiac specification and terminal differentiation. Although the diverse cardiac malformations associated with the loss of non-canonical Wnt11 in mice such as outflow tract (OFT) defects, reduced ventricular trabeculation, myofibrillar disorganization and reduced cardiac marker gene expression are well described, the underlying molecular mechanisms are still not completely understood. Here we aimed to further characterize Wnt11 mediated signal transduction during vertebrate cardiogenesis. Using Xenopus as a model system, we show by loss of function and corresponding rescue experiments that the non-canonical Wnt signalling mediator Rcsd1 is required downstream of Wnt11 for ventricular trabeculation, terminal differentiation of cardiomyocytes and cardiac morphogenesis. We here place Rcsd1 downstream of Wnt11 during cardiac development thereby providing a novel mechanism for how non-canonical Wnt signalling regulates vertebrate cardiogenesis. see all
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Series: |
Developmental biology |
ISSN: | 0012-1606 |
ISSN-E: | 1095-564X |
ISSN-L: | 0012-1606 |
Volume: | 242 |
Issue: | 1 |
Pages: | 28 - 39 |
DOI: | 10.1016/j.ydbio.2017.02.014 |
OADOI: | https://oadoi.org/10.1016/j.ydbio.2017.02.014 |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
1184 Genetics, developmental biology, physiology 3111 Biomedicine |
Subjects: | |
Funding: |
This work was supported by the Deutsche Forschungsgemeinschaft
DFG (SI-1381/1-2 to IOS and Ku1166/3-2 to MK). AH, MR and PRT
were supported by the International Graduate School in Molecular
Medicine of Ulm University (GSC270). |
http://dx.doi.org/10.1016/j.ydbio.2017.02.014 |
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Copyright information: |
© 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/) |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |