University of Oulu

Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Boris Baduev, Zhanna Shatilina, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev. Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers. Biology Open 2017 6: 673-677; doi: 10.1242/bio.024380

Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers

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Author: Borvinskaya, Ekaterina1,2; Gurkov, Anton1,3; Shchapova, Ekaterina1;
Organizations: 1Institute of Biology at Irkutsk State University, Irkutsk 664003, Russia
22Institute of Biology at Karelian Research Centre of Russian Academy of Sciences, Petrozavodsk 185035, Russia
3Baikal Research Centre, Irkutsk 664003, Russia
4Institute of Materials Research and Engineering, A*STAR, 138634, Singapore
5University of Oulu, Optoelectronics and Measurement Techniques Laboratory, Oulu 90570, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.9 MB)
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Language: English
Published: Company of Biologists, 2017
Publish Date: 2017-08-21


Tracking physiological parameters in different organs within the same organism simultaneously and in real time can provide an outstanding representation of the organism’s physiological status. The state-of-the-art technique of using encapsulated fluorescent molecular probes (microencapsulated biomarkers) is a unique tool that can serve as a platform for the development of new methods to obtain in vivo physiological measurements and is applicable to a broad range of organisms. Here, we describe a novel technique to monitor the pH of blood inside the gill capillaries and interstitial fluid of muscles by using microencapsulated biomarkers in a zebrafish model. The functionality of the proposed technique is shown by the identification of acidification under anesthesia-induced coma and after death. The pH in muscles reacts to hypoxia faster than that in the gill bloodstream, which makes both parameters applicable as markers of either local or bodily reactions.

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Series: Biology open
ISSN: 2046-6390
ISSN-E: 2046-6390
ISSN-L: 2046-6390
Volume: 6
Pages: 673 - 677
DOI: 10.1242/bio.024380
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Copyright information: © 2017. Published by The Company of Biologists Ltd is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.