University of Oulu

Korpi, R., Alestalo, K., Ruuska, T., Lammentausta, E., Borra, R., Yannopoulos, F., Lehtonen, S., Korpi, J., Lappi-Blanco, E., Anttila, V., Lehenkari, P., Juvonen, T., Blanco Sequieros, R. (2017) Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct. Acta Radiologica Open, 6 (8), 205846011771840. doi:10.1177/2058460117718407

Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct

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Author: Korpi, Riikka M1,2; Alestalo, Kirsi3,4; Ruuska, Timo4;
Organizations: 1Department of Diagnostic Radiology, University of Oulu and Oulu University Hospital
2Department of Radiology, Helsinki University Hospital
3Department of Surgery and Clinical Research Center, University of Oulu and Oulu University Hospital
4Department of Anatomy and Cell Biology, University of Oulu
5Medical Imaging Center of Southwest Finland, Turku University Hospital
6A.A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital
7MRC Oulu and Department of Obstetrics and Gynecology, Oulu University Hospital and PEDEGO Research Unit, University of Oulu
8Department of Otorhinolaryngology, Head and Neck Surgery, Helsinki University Hospital
9Department of Pathology, University of Oulu and Oulu University Hospital
10Department of Cardiac Surgery, HUCH Heart and Lung Center
11Department of Radiology, University of Turku and Turku University Hospital
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.6 MB)
Persistent link:
Language: English
Published: SAGE Publications, 2017
Publish Date: 2017-11-09


Background: Acute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. Cellular decay due hypoxia requires rapid and validated methods for possible therapeutic cell transplantation.

Purpose: To develop direct and rapid superparamagnetic iron oxide (SPIO) cell label for a large-animal model and to assess in vivo cell targeting by magnetic resonance imaging (MRI) in an experimental AMI model.

Material and Methods: Bone marrow mononuclear cells (BMMNCs) were labeled with SPIO particles using two novel direct labeling methods (rotating incubation method and electroporation). Labeling, iron incorporation in cells and label distribution, cellular viability, and proliferation were validated in vitro. An AMI porcine model was used to evaluate the direct labeling method (rotating incubation method) by examining targeting of labeled BMMNCs using MRI and histology.

Results: Labeling (1 h) did not alter either cellular differentiation potential or viability of cells in vitro. Cellular relaxation values at 9.4 T correlated with label concentration and MRI at 1.5 T showing 89 ± 4% signal reduction compared with non-labeled cells in vitro. In vivo, a high spatial correlation between MRI and histology was observed. The extent of macroscopic pathological myocardial changes (hemorrhage) correlated with altered function detected on MRI.

Conclusion: We demonstrated two novel direct SPIO labeling methods and demonstrated the feasibility of clinical MRI for monitoring targeting of the labeled cells in animal models of AMI.

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Series: Acta radiologica open
ISSN: 2058-4601
ISSN-E: 2058-4601
ISSN-L: 2058-4601
Volume: 6
Issue: 8
Article number: 2058460117718407
DOI: 10.1177/2058460117718407
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
3121 General medicine, internal medicine and other clinical medicine
Funding: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study has received funding from the Sigrid Jusélius Foundation.
Copyright information: © The Foundation Acta Radiologica 2017. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( which permits non-commercial use, reproduction and distribution of the work without further permiss ion provided the original work is attributed as specified on the SAGE and Open Access page (