University of Oulu

Al-Samadi, A., Adnan Awad, S., Tuomainen, K., Zhao, Y., Salem, A., Parikka, M., Salo, T. (2017) Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models. Oncotarget, 8 (36), 60123-60134. doi:10.18632/oncotarget.17768

Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models

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Author: Al-Samadi, Ahmed1; Awad, Shady Adnan2; Tuomainen, Katja1,3;
Organizations: 1Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki
2Hematology Research Unit, Department of Hematology, University of Helsinki and Helsinki University Central Hospital Comprehensive Cancer Center
3Department of Otorhinolaryngology, Helsinki University Hospital
4Department of Internal Medicine, Clinicum, University of Helsinki
5Faculty of Medicine and Life Sciences, University of Tampere
6Oral and Maxillofacial Unit, Tampere University Hospital
7Cancer and Translational Medicine Research Unit, University of Oulu
8Medical Research Center, Oulu University Hospital
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 6.9 MB)
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Language: English
Published: Impact Journals, 2017
Publish Date: 2017-11-28


The crosstalk between immune cells, cancer cells, and extracellular vesicles (EVs) secreted by cancer cells remains poorly understood. We created three-dimensional (3D) cell culture models using human leiomyoma discs and Myogel to study the effects of immune cells on highly (HSC-3) and less (SCC-25) invasive oral tongue squamous cell carcinoma (OTSCC) cell lines. Additionally, we studied the effects of EVs isolated from these cell lines on the cytotoxicity of CD8+ T and NK cells isolated from three healthy donors. Our analysis included the effects of these EVs on innate immunity in zebrafish larvae. Activated immune cells significantly decreased the proliferation of both OTSCC cell lines and associated with a diminished invasion area of HSC-3 cells. In general, EVs from SCC-25 increased the cytotoxic activity of CD8+ T and NK cells more than those from HSC-3 cells. However, this effect varied depending on the source and the immune and cancer cell subgroups. In zebrafish, the amount of IL-13 mRNA was decreased by SCC-25 EVs. This study describes promising in vitro and in vivo models to investigate interactions between immune cells, cancer cells, and EVs.

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Series: Oncotarget
ISSN: 1949-2553
ISSN-E: 1949-2553
ISSN-L: 1949-2553
Volume: 8
Issue: 36
Pages: 60123 - 60134
DOI: 10.18632/oncotarget.17768
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: This work was supported by the Sigrid Juselius Foundation, the Finnish Cancer Foundation, and the University of Helsinki Central Hospital.
Copyright information: Copyright: Al-Samadi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.