University of Oulu

de Andrade, A. L. D. L., de Oliveira, C. E., Dourado, M. R., Macedo, C. C. S., Winck, F. V., Paes Leme, A. F., Salo, T., Coletta, R. D., de AlmeidaFreitas, R. and Galvão, H. C. (), Extracellular vesicles from oral squamous carcinoma cells display pro- and antiangiogenic properties. Oral Dis. Accepted Author Manuscript. doi:10.1111/odi.12765

Extracellular vesicles from oral squamous carcinoma cells display pro- and antiangiogenic properties

Saved in:
Author: Dias Leite de Andrade, Ana Luiza1,2; Ervolino de Oliveira, Carine2; Rocha Dourado, Maurício2;
Organizations: 1Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil
2Department of Oral Diagnosis, School of Dentistry, University of Campinas, Piracicaba, SP, Brazil
3Mass Spectrometry Laboratory, Biosciences National Laboratory, LNBio, CNPEM, Campinas, SP, Brazil
4Department of Diagnostics and Oral Medicine, Institute of Dentistry and Oulu University Hospital and Medical Research Center, University of Oulu, Oulu, Finland; and Institute of Dentistry, University of Helsinki, Helsinki, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2017113055178
Language: English
Published: John Wiley & Sons, 2017
Publish Date: 2018-09-08
Description:

Abstract

Background: A new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completed understood. Here we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis.

Methods: OSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot-blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs.

Results: The results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation.

Conclusions: The findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or antiangiogenic effects.

see all

Series: Oral diseases
ISSN: 1354-523X
ISSN-E: 1601-0825
ISSN-L: 1354-523X
Volume: Accepted article
DOI: 10.1111/odi.12765
OADOI: https://oadoi.org/10.1111/odi.12765
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Funding: The authors would like to thank the Procad/Casadinho-CNPq/CAPES, Brasília, Brazil, and the Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP, São Paulo, Brazil (2013/19856-2) for their financial support of this research.
Copyright information: This is the peer reviewed version of the following article: de Andrade, A. L. D. L., de Oliveira, C. E., Dourado, M. R., Macedo, C. C. S., Winck, F. V., Paes Leme, A. F., Salo, T., Coletta, R. D., de AlmeidaFreitas, R. and Galvão, H. C. (), Extracellular vesicles from oral squamous carcinoma cells display pro- and antiangiogenic properties. Oral Dis. Accepted Author Manuscript. doi:10.1111/odi.12765, which has been published in final form at http://dx.doi.org/10.1111/odi.12765. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.