University of Oulu

Gurkov A, Sadovoy A, Shchapova E, Teh C, Meglinski I, Timofeyev M (2017) Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos. PLoS ONE 12(10): e0186548. https://doi.org/10.1371/journal.pone.0186548

Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos

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Author: Gurkov, Anton1; Sadovoy, Anton2; Shchapova, Ekaterina1;
Organizations: 1Institute of Biology, Irkutsk State University
2Institute of Materials Research and Engineering, Agency for Science, Technology and Research, Singapore
3Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore
4Optoelectronics and Measurement Techniques Laboratory, University of Oulu
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.9 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2017120555293
Language: English
Published: Public Library of Science, 2017
Publish Date: 2017-12-05
Description:

Abstract

In vivo physiological measurement is a major challenge in modern science and technology, as is environment conservation at the global scale. Proper toxicological testing of widely produced mixtures of chemicals is a necessary step in the development of new products, allowing us to minimize the human impact on aquatic ecosystems. However, currently available bioassay-based techniques utilizing small aquatic organisms such as fish embryos for toxicity testing do not allow assessing in time the changes in physiological parameters in the same individual. In this study, we introduce microencapsulated fluorescent probes as a promising tool for in vivo monitoring of internal pH variation in zebrafish embryos. The pH alteration identified under stress conditions demonstrates the applicability of the microencapsulated fluorescent probes for the repeated analysis of the embryo’s physiological state. The proposed approach has strong potential to simultaneously measure a range of physiological characteristics using a set of specific fluorescent probes and to finally bring toxicological bioassays and related research fields to a new level of effectiveness and sensitivity.

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Series: PLoS one
ISSN: 1932-6203
ISSN-E: 1932-6203
ISSN-L: 1932-6203
Volume: 12
Issue: 10
Article number: e0186548
DOI: 10.1371/journal.pone.0186548
OADOI: https://oadoi.org/10.1371/journal.pone.0186548
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
1172 Environmental sciences
Subjects:
Funding: This work was supported by the Agency for Science, Technology and Research of Singapore (A*STAR); the Russian Science Foundation (grant #15-14-10008); «Contact Singapore»’s program «Experience@Singapore: Summer Research Internship 2013» and by Singapore International Pre-Graduate Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Copyright information: © 2017 Gurkov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  https://creativecommons.org/licenses/by/4.0/