University of Oulu

Gomes-Silva W, Prado-Ribeiro A, C, Brandão T, B, Morais-Faria K, de Castro Junior G, Mak M, P, Lopes M, A, Rocha M, M, Salo T, Tjäderhane L, de Goes M, F, Santos-Silva A, R, Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries. Caries Res 2017;51:216-224

Postradiation matrix metalloproteinase-20 expression and its impact on dental micromorphology and radiation-related caries

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Author: Gomes-Silva, Wagner1,2; Prado-Ribeiro, Ana Carolina1,2; Brandão, Thais Bianca1,2;
Organizations: 1Oral Diagnosis Department, Piracicaba Dental School, University of Campinas, Piracicaba
2Dental Oncology Service, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Sao Paulo, Brazil
3Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Sao Paulo, Brazil
4 Clinical Oncology Department, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Sao Paulo, Brazil
5Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu
6Medical Research Center Oulu
7Department of Oral and Maxillofacial Diseases, University of Helsinki
8 Laboratory of Helsinki University Central Hospital
9 Research Unit of Oral Health Sciences, Oulu University Hospital and University of Oulu, Oulu
10Helsinki University Hospital, Helsinki , Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2017120855521
Language: English
Published: Karger, 2017
Publish Date: 2018-03-31
Description:

Abstract

Recent evidence suggests that head-and-neck radiotherapy (HNRT) increases active forms of matrix metalloproteinase-20 (MMP-20) in human tooth crowns, degrading the dentin-enamel junction (DEJ) and leading to enamel delamination, which is a pivotal step in the formation of radiation-related caries (RRC). Additional participation of enzymatic degradation of organic matrix components in caries progression was attributed to MMP-20 in dentin. Therefore, the current study tested the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes to the enamel and dentin. Thirty-six teeth were studied, including 19 post-HNRT specimens and 17 nonirradiated controls. Optical light microscopy was used to investigate the micromorphological components of the DEJ, dentin-pulp complex components, and carious dentin. The samples were divided into 2 subgroups: nondemineralized ground sections (n = 20) and demineralized histological sections (n = 16). In addition, immunohistochemical analysis using the immunoperoxidase technique was conducted to semiquantitatively assess MMP-20 expression in the DEJ, dentin-pulp complex components, and carious dentin. No apparent damage to the DEJ microstructure or other dentin-pulp complex components was observed and no statistically significant differences were detected in MMP-20 expression (p > 0.05) between the irradiated and control groups. This study rejected the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes. Hence, direct effects of radiation may not be regarded as an independent factor to explain aggressive clinical patterns of RRC.

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Series: Caries research
ISSN: 0008-6568
ISSN-E: 1421-976X
ISSN-L: 0008-6568
Volume: 51
Issue: 3
Pages: 216 - 224
DOI: 10.1159/000457806
OADOI: https://oadoi.org/10.1159/000457806
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Funding: The authors would like to gratefully acknowledge the financial support of the São Paulo Research Foundation (FAPESP, process No. 2016/22059-5, 2013/18402-8, and 2012/06138-1). Thanks also go to the International Relations Office of University of Campinas (VRERI, process No. 045/2015).
Copyright information: © 2017 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: Gomes-Silva W, Prado-Ribeiro A, C, Brandão T, B, Morais-Faria K, de Castro Junior G, Mak M, P, Lopes M, A, Rocha M, M, Salo T, Tjäderhane L, de Goes M, F, Santos-Silva A, R, Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries. Caries Res 2017;51:216-224. The final, published version is available at http://www.karger.com/?doi=10.1159/000457806.