University of Oulu

Johanna Korvala, Kowan Jee, Emmi Porkola, Alhadi Almangush, Neda Mosakhani, Carolina Bitu, Nilva K. Cervigne, Flávia S. Zandonadi, Gabriela V. Meirelles, Adriana Franco Paes Leme, Ricardo D. Coletta, Ilmo Leivo, Tuula Salo, MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro, In Experimental Cell Research, Volume 350, Issue 1, 2017, Pages 9-18, ISSN 0014-4827, (

MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

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Author: Korvala, Johanna1; Jee, Kowan2,3; Porkola, Emmi1;
Organizations: 1Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland
2Department of Pathology, University of Turku, Turku University Hospital, Turku, Finland
3Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
4Department of Pathology, HUSLAB, Helsinki, Finland
5Department of Oral Diagnosis, School of Dentistry, University of Campinas (UNICAMP), Av. Limeira, 901 – Bairro Areião, CEP: 13414-903 Piracicaba, São Paulo, Brazil
6Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Brazil
7Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo, Brazil
8Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
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Language: English
Published: Elsevier, 2017
Publish Date: 2017-12-11


Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.

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Series: Experimental cell research
ISSN: 0014-4827
ISSN-E: 1090-2422
ISSN-L: 0014-4827
Volume: 350
Issue: 1
Pages: 9 - 18
DOI: 10.1016/j.yexcr.2016.10.015
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: This study was supported by the Finnish Cancer Society (TS, IL, KJ), The Sigrid Juselius Foundation (TS), Oulu University Hospital MRC and VTR funding (TS), The Finska Läkaresällskapet foundation (IL, KJ), The Maritza and Reino Salonen Foundation (IL, KJ), and the Turku University Hospital VTR funding (RG). JK was supported by the Finnish Cultural Foundation, the Thelma Mäkikyrö Foundation and Science without borders (CAPES, Brazil). AFPL was supported by FAPESP (2009/54067-3 and 2010/19278-0) and CNPq grants (470268/2013-1). RDC was supported by Science without borders (CAPES, Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES, Brasília, Brazil (AUXPE-PVES-570/2013) and Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP, São Paulo, Brazil (2013/01607-6).
Copyright information: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license