Cross-reactive saliva IgA antibodies to oxidized LDL and periodontal pathogens in humans
|Author:||Akhi, Ramin1,2,3,4; Wang, Chunguang1,2,3; Kyrklund, Mikael1,2,3;|
1Medical Microbiology and Immunology, Research Unit of Biomedicine, University of Oulu, Finland
2Medical Research Center, Oulu University Hospital and University of Oulu, Finland
3Nordlab, Oulu University Hospital, Finland
4Research Unit of Oral Health Sciences, University of Oulu, Finland
5Research Programs Unit, Genome-Scale Biology, University of Helsinki, Helsinki, Finland
6Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland
7Cancer Research and Translational Medicine Research Unit, University of Oulu, Finland
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2017122156007
John Wiley & Sons,
|Publish Date:|| 2018-06-29
Aim: Oxidized low-density lipoproteins (oxLDL) are formed as a result of lipid peroxidation and are highly immunogenic and proatherogenic. In this study, saliva antibodies binding to oxLDL, Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) were characterized and their cross-reactivity was evaluated.
Materials and Methods: Resting and stimulated saliva samples were collected from 36 healthy adults (mean age 26 years). Saliva IgA, IgG and IgM autoantibody levels to copper oxidized LDL (CuOx-LDL) and malondialdehyde acetaldehyde-modified LDL (MAA-LDL) were determined with chemiluminescence immunoassay.
Results: Saliva IgA and IgG antibodies binding to MAA-LDL and CuOx-LDL were detected in all samples and they were associated with the saliva levels of IgA and IgG to P. gingivalis and A. actinomycetemcomitans. Competitive immunoassay showed that saliva antibodies to MAA-LDL cross-reacted specifically with P. gingivalis. The autoantibody levels to oxLDL in saliva were not associated with the autoantibody levels to oxLDL in plasma or with saliva apolipoprotein B 100 levels.
Conclusions: Saliva contains IgA and IgG binding to oxLDL, which showed cross-reactive properties with the periodontal pathogens Porphyromonas gingivalis (P.g). The data suggest that secretory IgA to P.g may participate in immune reactions involved in LDL oxidation through molecular mimicry.
Journal of clinical periodontology
|Pages:||682 - 691|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
The authors declare that they have no conflicts of interest. The study was supported by the Sigrid Juselius Foundation (S.H.), the Finnish Foundation for Cardiovascular Research (S.H. and O.K.), the Academy of Finland (1266053; P.J.P.) and the Wihuri Foundation (R.A.).
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Akhi R, Wang C, Kyrklund M, et al. Cross-reactive saliva IgA antibodies to oxidized LDL and periodontal pathogens in humans. J Clin Periodontol. 2017;44:682–691. https://doi.org/10.1111/jcpe.12748, which has been published in final form at https://doi.org/10.1111/jcpe.12748. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.