University of Oulu

Haapalainen, A. M., Karjalainen, M. K., Daddali, R., Ohlmeier, S., Anttonen, J., Määttä, T. A., Salminen, A., Mahlman, M., Bergmann, U., Mäkikallio, K., Ojaniemi, Haapalainen, A.M., Karjalainen, M.K., Daddali, R., Ohlmeier, S., Anttonen, J., Määttä, T.A., Salminen, A., Mahlman, M., Bergmann, U., Mäkikallio, K., Ojaniemi, M., Hallman, M. and Rämet, M. (2018), Expression of CPPED1 in human trophoblasts is associated with timing of term birth. J. Cell. Mol. Med., 22: 968-981.

Expression of CPPED1 in human trophoblasts is associated with timing of term birth

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Author: Haapalainen, Antti M.1,2; Karjalainen, Minna K.1,2; Daddali, Ravindra1,2;
Organizations: 1PEDEGO Research Unit and Medical Research Center Oulu, University of Oulu, Oulu, Finland
2Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland
3Proteomics Core Facility, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
4Department of Obstetrics and Gynecology, Turku University Hospital and University of Turku, Turku, Finland
5BioMediTech Institute and Faculty of Medical and Life Sciences, University of Tampere, Tampere, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.8 MB)
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Language: English
Published: John Wiley & Sons, 2018
Publish Date: 2018-01-05


Understanding of timing of human parturition is incomplete. Therefore, we carried out proteomic analyses of full-term placentas from uncomplicated pregnancies to identify protein signatures associated with the onset of spontaneous delivery. We found quantitative associations of 10 proteins with spontaneous term birth, evident either in the basal or in the chorionic plates or in both. Additional 18 proteins were associated according to the location within placenta indicating local variations in protein amounts. Calcineurin-like phosphoesterase domain-containing 1 (CPPED1), a phosphatase previously suggested dephosphorylating AKT1/PKB, was one of the identified proteins. qRT-PCR revealed the mRNA level of CPPED1 was higher in elective caesarean deliveries than in spontaneous births, while immunohistochemistry showed CPPED1 in cytotrophoblasts, syncytiotrophoblasts and extravillous trophoblasts. Noteworthy, phosphorylation status of AKT1 did not differ between placentas from elective caesarean and spontaneous deliveries. Additionally, analyses of samples from infants indicated that single-nucleotide polymorphisms rs11643593 and rs8048866 of CPPED1 were associated with duration of term pregnancy. Finally, post-transcriptional silencing of CPPED1 in cultured HTR8/SVneo cells by siRNAs affected gene expression in pathways associated with inflammation and blood vessel development. We postulate that functions regulated by CPPED1 in trophoblasts at choriodecidual interphase have a role in the induction of term labour, but it may be independent of AKT1.

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Series: Journal of cellular and molecular medicine
ISSN: 1582-1838
ISSN-E: 1582-4934
ISSN-L: 1582-1838
Volume: 22
Issue: 2
Pages: 968 - 981
DOI: 10.1111/jcmm.13402
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
1184 Genetics, developmental biology, physiology
Funding: This work was supported by the Jane and Aatos Erkko Foundation (MH and MR), Sigrid Juselius Foundation (MH), Competitive State Research Financing of the Expert Responsibility Area of Oulu University Hospital (MR), Emil Aaltonen Foundation (MO), KA Snellman Foundation (MO and MR) and Pediatric Research Foundation (AMH).
Copyright information: © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.