University of Oulu

Tolonen, Tiina; von Wright, Atte; Virtanen, Vesa (2017) Chemical composition of the water extract of young spruce (Picea abies (L.) Karst) shoots. In: Rainer Peltonen (Ed.) Conference on Non-Timber Forest Products and Bioeconomy, 28-30 November 2017, Rovaniemi, Finland, (pp. 65), Natural resources and bioeconomy studies 72/2017

Chemical composition of the water extract of young spruce (Picea abies (L.) Karst) shoots

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Author: Tolonen, Tiina1; von Wright, Atte2; Virtanen, Vesa1
Organizations: 1Unit of Measurement Technology, Kajaani University Consortium, University of Oulu, Kajaani, Finland
2Food Biotechnology, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland
Format: abstract
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.1 MB)
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Language: English
Published: Luonnonvarakeskus, 2017
Publish Date: 2018-01-22


Young shoots of spruce are a natural resource that have been used as a remedy or a food ingredient for a long time. Although, it cannot be harvested from the forest without the permission of the landowner, it could be exploited more. Almost third of Finnish forests resources is spruce, forming enormous reservoir for the spruce shoots and more refined spruce shoot products.

Lactic acid bacteria is one of the most, if not the most, used group of microbes in the food industry. In our earlier growth experiments the water extract from young spruce shoots had an enhancing effects in the growth of some strains of Lactobacillus species. Particularly, Lactobacillus plantarum grew better and had shorter lag time, when growth medium contained spruce shoot extract. To apprehend better, what could generate an effect seen with the growth of the bacteria, we analyzed the chemical composition of the water extract of the spruce shoots.

For the evaluation of the chemical composition of the spruce shoot water extract chromatographic, capillary electrophoretic and spectrophotometric methods were used. Sugars and sugar alcohols were measured with the capillary electrophoresis (CE), glucose and inositol respectively being of most abundant. Also non phenolic acids and inorganic cations were analyzed with the CE. Quinic acid was the main non phenolic acid detected, and of the inorganic cations K, Mg, Ca, Ni, Zn and ammonium were recognized from the extract.

Antioxidant capacity (reduction potential) of 772mg GAE/L was measured for the extract using spectrophotometric method of Folin-Ciocalteu. To analyze phenolic compounds, chromatographic methods developed for the detection of catechins, proantocyanidin, phenolic acids and stilbenoids were utilized. Main catechins in the water extract were the catechin and epicatechin. Type B dimers were the most apparent of the proantocyanidins. The extract contained several benzoic and cinnamic acid derivatives, but protocatechuic acid and p-coumaric acid were the most apparent ones. Of the stilbenoids trans and cis forms of piceid, sugar derivative of resveratrol, and astringin were the most abundant.

Future research will focus on, whether Lactobacillus bacteria will metabolize one or more of these detected compounds found when in growth with the extract.

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Series: Luonnonvara- ja biotalouden tutkimus
ISSN: 2342-7647
ISSN-E: 2342-7639
ISSN-L: 2342-7647
ISBN: 978-952-326-493-9
ISBN Print: 978-952-326-492-2
Volume: 72/2017
Pages: 65 - 65
Article number: P26
Host publication: Conference on Non-Timber Forest Products and Bioeconomy, 28-30 November 2017, Rovaniemi, Finland
Host publication editor: Peltola, Rainer
Conference: Conference on Non-Timber Forest Products and Bioeconomy
Field of Science: 4112 Forestry
116 Chemical sciences
Copyright information: © 2017 The Authors and Natural Resources Institute Finland. Published in this repository with the kind permission of the publisher.