University of Oulu

Nelli Roininen, Kirsi-Maria Haapasaari, and Peeter Karihtala, “The Role of Redox-Regulating Enzymes in Inoperable Breast Cancers Treated with Neoadjuvant Chemotherapy,” Oxidative Medicine and Cellular Longevity, vol. 2017, Article ID 2908039, 9 pages, 2017. doi:10.1155/2017/2908039

The role of redox-regulating enzymes in inoperable breast cancers treated with neoadjuvant chemotherapy

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Author: Roininen, Nelli1,2; Haapasaari, Kirsi-Maria2; Karihtala, Peeter1
Organizations: 1Department of Oncology and Radiotherapy, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland
2Department of Pathology, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 15.3 MB)
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Language: English
Published: Hindawi, 2017
Publish Date: 2018-01-30


Although validated predictive factors for breast cancer chemoresistance are scarce, there is emerging evidence that the induction of certain redox-regulating enzymes may contribute to a poor chemotherapy effect. We investigated the possible association between chemoresistance and cellular redox state regulation in patients undergoing neoadjuvant chemotherapy (NACT) for breast cancer. In total, 53 women with primarily inoperable or inflammatory breast cancer who were treated with NACT were included in the study. Pre-NACT core needle biopsies and postoperative tumor samples were immunohistochemically stained for nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), thioredoxin (Trx), and peroxiredoxin I (Prx I). The expression of all studied markers increased during NACT. Higher pre-NACT nuclear Prx I expression predicted smaller size of a resected tumor (p = 0 00052; r = −0 550), and higher pre-NACT cytoplasmic Prx I expression predicted a lower amount of evacuated nodal metastasis (p = 0 0024; r = −0 472). Pre-NACT nuclear Trx expression and pre-NACT nuclear Keap1 expression had only a minor prognostic significance as separate factors, but when they were combined, low expression for both antibodies before NACT predicted dismal disease-free survival (log-rank p = 0 0030). Our results suggest that redox-regulating enzymes may serve as potential prognostic factors in primarily inoperable breast cancer patients.

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Series: Oxidative medicine and cellular longevity
ISSN: 1942-0900
ISSN-E: 1942-0994
ISSN-L: 1942-0900
Volume: 2017
Article number: 2908039
DOI: 10.1155/2017/2908039
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: The Heikki Ruotsalainen and Thelma Mäkikyrö Foundations provided funding for the study.
Copyright information: Copyright © 2017 Nelli Roininen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.