University of Oulu

Sova SS, Tjäderhane L, Heikkilä PA and Jernvall J (2018) A microCT Study of Three-Dimensional Patterns of Biomineralization in Pig Molars. Front. Physiol. 9:71. doi: 10.3389/fphys.2018.00071

A microCT study of three-dimensional patterns of biomineralization in pig molars

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Author: Sova, Susanna S.1,2; Tjäderhane, Leo3,4; Heikkilä , Pasi A.2;
Organizations: 1Developmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, Finland
2Department of Geoscience and Geography, University of Helsinki, Helsinki, Finland
3Department of Oral and Maxillofacial Diseases, Helsinki University Hospital, University of Helsinki, Helsinki, Finland
4Institute of Dentistry and Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 5.3 MB)
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Language: English
Published: Frontiers Media, 2018
Publish Date: 2018-04-12


Domestic pig molars provide an interesting system to study the biomineralization process. The large size, thick enamel and complex crown morphology make pig molars relatively similar to human molars. However, compared to human molars, pig molars develop considerably faster. Here we use microCT to image the developing pig molars and to decipher spatial patterns of biomineralization. We used mineral grains to calibrate individual microCT-scans, which allowed an accurate measure of the electron density of the developing molars. The microCT results show that unerupted molars that are morphologically at the same stage of development, can be at markedly different stage of enamel biomineralization. Erupted molars show increased electron density, suggesting that mineralization continues in oral cavity. Yet, our comparisons show that human enamel has slightly higher electron density than pig enamel. These results support the relatively low hardness values and calcium level values that have been reported earlier in literature for pig teeth. The mineral calibration was an efficient method for the microCT-absorption models, allowing a relatively robust way to detect scanning artifacts. In conclusions, whereas thin sections remain the preferred way to analyze enamel features, such as incremental lines and crystal orientation, the microCT allows efficient and non-destructive comparisons between different teeth and species.

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Series: Frontiers in physiology
ISSN: 1664-042X
ISSN-E: 1664-042X
ISSN-L: 1664-042X
Volume: 9
Pages: 1 - 7
Article number: 71
DOI: 10.3389/fphys.2018.00071
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Copyright information: © 2018 Sova, Tjäderhane, Heikkilä and Jernvall. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.