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Site-specific glycosaminoglycan content is better maintained in the pericellular matrix than the extracellular matrix in early post-traumatic osteoarthritis |
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| Author: | Ojanen, Simo P.1,2; Finnilä, Mikko A. J.1,2; Reunamo, Aino E.1; |
| Organizations: |
1Department of Applied Physics, University of Eastern Finland 2Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu 3Mechanical & Manufacturing Engineering, Schulich School of Engineering, University of Calgary
4Human performance laboratory, Faculty of Kinesiology, University of Calgary
5Department of Diagnostic Radiology, Oulu University Hospital |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 10.8 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe2018060425239 |
| Language: | English |
| Published: |
Public Library of Science,
2018
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| Publish Date: | 2018-06-04 |
| Description: |
AbstractIntroduction: One of the characteristics of early osteoarthritis (OA) is the loss of fixed charged density (FCD) of glycosaminoglycans in the superficial zone of articular cartilage. However, possible local changes in the FCD content of the pericellular matrix (PCM) are not fully understood. Hence, our aim was to investigate the effect of unilateral anterior cruciate ligament transection (ACLT) in rabbit knees on estimated FCD in the PCM compared to that in the ECM, and relate these results with cell morphology. Methods: Articular cartilage samples were collected from ACLT, contralateral and intact control knee joints from lateral and medial femoral condyles and tibial plateaus, and from the femoral groove and patella. Histological samples were prepared and stained with Safranin-O to estimate the FCD content around the chondrocytes in the PCM and the ECM with digital densitometry. Results: As a result of ACLT, the greatest decreases in the FCD content of the PCM were observed in the superficial zone of the lateral femoral condyle (p = 0.02), medial tibial plateau (p = 0.002) and patellar (p < 0.001) cartilage. The normalized FCD content of the PCM compared to the surrounding ECM was increased most in the femoral condyles (p < 0.01) and medial tibial plateau (p = 0.02) cartilage. The high normalized FCD content of the PCM in the superficial zone of lateral femoral condyle cartilage was consistent with the round cell morphology in that location. Conclusions: In conclusion, we suggest that certain sites in the knee joint, particularly the lateral femoral condyle cartilage, experience less FCD loss in the PCM than in the ECM in early post-traumatic OA, which could lead to altered cell shape. see all
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| Series: |
PLoS one |
| ISSN: | 1932-6203 |
| ISSN-E: | 1932-6203 |
| ISSN-L: | 1932-6203 |
| Volume: | 13 |
| Issue: | 4 |
| Article number: | e0196203 |
| DOI: | 10.1371/journal.pone.0196203 |
| OADOI: | https://oadoi.org/10.1371/journal.pone.0196203 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
3111 Biomedicine |
| Subjects: | |
| Funding: |
This work was supported by the following: European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement no. 336267, PhD Simo Saarakkala; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Academy of Finland, grant nos. 268378 and 303786, PhD Simo Saarakkala; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Sigrid Juselius Foundation, PhD Simo Saarakkala; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Strategic funding from the University of Oulu, PhD Simo Saarakkala; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement no. 281180, PhD Rami Korhonen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Academy of Finland, grant no. 286526, PhD Rami Korhonen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Sigrid Juselius Foundation, PhD Rami Korhonen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Päivikki and Sakari Sohlberg Foundation, Mr. Simo Ojanen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Pohjois-Savon Rahasto; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Canadian Institutes of Health Research (CIHR), PhD Walter Herzog; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Killam Foundation, PhD Walter Herzog; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Canada Research Chair Program, PhD Walter Herzog; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Maj ja Tor Nesslingin Säätiö (FI), PhD Santtu Mikkonen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Academy of Finland, grant no. 307331, PhD Santtu Mikkonen; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Strategic funding from the University of Eastern Finland, PhD Mikko Finnilä; The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| EU Grant Number: |
(336267) 3D-OA-HISTO - Development of 3D Histopathological Grading of Osteoarthritis |
| Academy of Finland Grant Number: |
268378 303786 286526 307331 |
| Detailed Information: |
268378 (Academy of Finland Funding decision) 303786 (Academy of Finland Funding decision) 286526 (Academy of Finland Funding decision) 307331 (Academy of Finland Funding decision) |
| Copyright information: |
© 2018 Ojanen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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https://creativecommons.org/licenses/by/4.0/ |