University of Oulu

Petri Tervasmäki, Marko Latva-Kokko, Sanna Taskila, Juha Tanskanen, Effect of oxygen transfer on yeast growth — Growth kinetic and reactor model to estimate scale-up effects in bioreactors, Food and Bioproducts Processing, Volume 111, 2018, Pages 129-140, ISSN 0960-3085,

Effect of oxygen transfer on yeast growth : growth kinetic and reactor model to estimate scale-up effects in bioreactors

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Author: Tervasmäki, Petri1; Latva-Kokko, Marko2; Taskila, Sanna1;
Organizations: 1University of Oulu, Chemical Process Engineering, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland
2Outotec Oyj, Outotec Research Center, P.O. Box 69, FI-23101 Pori, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.7 MB)
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Language: English
Published: Elsevier, 2018
Publish Date: 2020-08-14


Large scale fermentations face challenges in mixing and mass transfer as well as in the design and construction of the equipment. Scale-up from laboratory and pilot scale experiments is difficult because different phenomena — such as mixing times and mass transfer conditions — scale in a different way.

We study the effect of mass transfer, reactor type and scale on the growth of Pichia pastoris yeast. Batch cultivation experiments monitoring the cell growth and ethanol formation are conducted in laboratory scale in two reactor types — stirred tank and an Outotec OKTOP®9000 draft tube reactor. Model for the yeast growth — including respirative and fermentative metabolism and the effect of dissolved oxygen — is formed based on literature. For scale-up studies, the growth model is used along with one dimensional reactor model that accounts for liquid mixing, gas phase dynamics and local gas hold-up and mass transfer coefficient.

By using a realistic growth model along with the reactor model, the simulated effects of scale-up are presented in terms of cell yield. A decrease in yield is noticed due to oxygen depletion in gas and insufficient liquid mixing. Potential improvements are related to the gas handling capacity and liquid mixing of the reactor.

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Series: Food and bioproducts processing
ISSN: 0960-3085
ISSN-E: 0960-3085
ISSN-L: 0960-3085
Volume: 111
Pages: 129 - 140
DOI: 10.1016/j.fbp.2018.08.004
Type of Publication: A1 Journal article – refereed
Field of Science: 215 Chemical engineering
220 Industrial biotechnology
Funding: The authors acknowledge the Finnish Funding Agency forInnovation Tekes for providing financing for this work (Grantnumber 906/312016, Tackling mass transfer challenges in fer-mentations).
Copyright information: © 2018 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license