University of Oulu

Panjan, P., Virtanen, V., Sesay, A. (2018) Towards microbioprocess control: an inexpensive 3D printed microbioreactor with integrated online real-time glucose monitoring. Analyst, 143 (16), 3926-3933. doi:10.1039/C8AN00308D

Towards microbioprocess control : an inexpensive 3D printed microbioreactor with integrated online real-time glucose monitoring

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Author: Panjan, Peter1; Virtanen, Vesa1; Sesay, Adama Marie1
Organizations: 1Measurement Technology Unit (MITY), University of Oulu
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.3 MB)
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Language: English
Published: Royal Society of Chemistry, 2018
Publish Date: 2018-09-27


Bioprocessing is of crucial importance in pharmaceutical, biofuel, food and other industries. Miniaturization of bioprocesses into microbioreactors allows multiplexing of experiments as well as reduction of reagent consumption and labour-intensity. A crucial part of the research within microbioreactors is biochemical analysis of product, byproduct and substrate concentrations that currently heavily relies on large analytical equipment. Biosensors are a promising analytical tool, however, integration into a microbioreactor is associated with challenges in ensuring sterility, appropriate sensing range, control of matrix effects and stability. In this work we present a novel biosensor integrated analytical chip that features an internal, actuated buffer flow in contact with a biosensor downstream and a diffusion limiting membrane exposed to the sample upstream. The technology was developed and tested using an electrochemical glucose oxidase biosensor and was found to successfully surmount the aforementioned challenges including the extension of the linear range of sensitivity to more than 20 g L⁻¹ for online, real time monitoring of glucose. The biosensor integration chip with the glucose biosensor was then mounted onto a 3D printed microbioreactor with 1 mL of internal volume. The system successfully monitored the consumption of glucose of Saccharomyces cerevisiae in real time for more than 8 h. The developed technology and measurement methodologies are transferrable to other biosensors and microbioreactors as well as large scale applications.

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Series: Analyst
ISSN: 0003-2654
ISSN-E: 1364-5528
ISSN-L: 0003-2654
Volume: 143
Issue: 16
Pages: 3926 - 3933
DOI: 10.1039/C8AN00308D
Type of Publication: A1 Journal article – refereed
Field of Science: 220 Industrial biotechnology
Funding: This work is a part of the EuroMBR project, funded by the People Programme (Marie Curie Actions, Multi-ITN) of the European Union’s Seventh Framework Programme for research, technological development and demonstration project: The European network for innovative micro-bioreactor applications in ioprocess development (EUROMBR, Grant No. 608104).
EU Grant Number: (608104) EUROMBR - European network for innovative microbioreactor applications in bioprocess development
Copyright information: This journal is © The Royal Society of Chemistry 2018. This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence.