University of Oulu

Dmitry Vladimirovich Gradov, Mei Han, Petri Tervasmäki, Marko Latva-Kokko, Johanna Vaittinen, Arto Pihlajamäki, and Tuomas Koiranen, Numerical Simulation of Biomass Growth in OKTOP®9000 Reactor at Industrial Scale, Industrial & Engineering Chemistry Research 2018 57 (40), 13300-13311, DOI: 10.1021/acs.iecr.8b02765

Numerical simulation of biomass growth in OKTOP®9000 reactor at industrial scale

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Author: Gradov, Dmitry Vladimirovich1; Han, Mei1; Tervasmäki, Petri2;
Organizations: 1School of Engineering Science, Lappeenranta University of Technology, P.O. Box 20, FI-53851 Lappeenranta, Finland
2Chemical Process Engineering, University of Oulu, P.O. Box 4000, FI-90014 Oulu, Finland
3Outotec (Finland) Oy, Outotec Research Center, P.O. Box 69, FI-23101 Pori, Finland
4Neste Engineering Solutions, NAPCON, P.O. Box 310, FI-06101 Porvoo, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 6.1 MB)
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Language: English
Published: American Chemical Society, 2018
Publish Date: 2018-10-16


Computational fluid dynamics is a powerful method for scale-up of reactors although it is still challenging to fully embrace hydrodynamics and biological complexities. In this article, an aerobic fermentation of Pichia pastoris cells is modeled in a batch OKTOP®9000 reactor. The 800 m³ industrial scale reactor is equipped with a radial impeller, designed by Outotec Oy for gas dispersion in the draft tube reactor. Measured Np of the impeller is used in hydrodynamics validation. The resolved energy dissipation rate is compensated, and its influence on mass transfer is analyzed and discussed. Gas–liquid drag force is modified to simulate effects of liquid turbulence and bubble swarms. Resolved steady state multiphase hydrodynamics is used to simulate the fermentation process. Temporal evolution of species concentrations is compared to experimental data measured in a small copy of the reactor at lab scale (14 L). The effect of oxygenation on the P. pastoris cells cultivation is considered.

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Series: Industrial & engineering chemistry research
ISSN: 0888-5885
ISSN-E: 1520-5045
ISSN-L: 0888-5885
Volume: 57
Issue: 40
Pages: 13300 - 13311
DOI: 10.1021/acs.iecr.8b02765
Type of Publication: A1 Journal article – refereed
Field of Science: 215 Chemical engineering
220 Industrial biotechnology
Funding: The authors are grateful to the Finnish Funding Agency TEKES and, in particular, to Outotec Finland Oy and Neste Engineering Solutions Oy collaborating under FERMATRA project (908/31/2016 and 958/31/2016), for active supervision and financial support.
Copyright information: © 2018 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.