University of Oulu

Strniša, F., Bajić, M., Panjan, P., Plazl, I., Sesay, A., Žnidaršič-Plazl, P. (2018) Characterization of an enzymatic packed-bed microreactor: Experiments and modeling. Chemical Engineering Journal, 350, 541-550. doi:10.1016/j.cej.2018.05.028

Characterization of an enzymatic packed-bed microreactor : experiments and modeling

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Author: Strniša, Filip1; Bajić, Marijan1; Panjan, Peter2;
Organizations: 1Faculty of Chemistry and Chemical Technology, University of Ljubljana
2Measurement Technology Unit, University of Oulu
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.8 MB)
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Language: English
Published: Elsevier, 2018
Publish Date: 2020-05-05


A micro packed-bed reactor (µPBR) based on two-parallel-plates configuration with immobilized Candida antarctica lipase B in the form of porous particles (Novozym® 435) was theoretically and experimentally characterized. A residence time distribution (RTD) within µPBRs comprising various random distributions of particles placed in one layer was computationally predicted by a mesoscopic lattice Boltzmann (LB) method. Numerical simulations were compared with measurements of RTD, obtained by stimulus-response experiment with a pulse input using glucose as a tracer, monitored by an electrochemical glucose oxidase microbiosensor integrated with the reactor. The model was validated by a good agreement between the experimental data and predictions of LB model at different conditions. The developed µPBR was scaled-up in length and width comprising either a single or two layers of Novozym® 435 particles and compared regarding the selected enzyme-catalyzed transesterification. A linear increase in the productivity with the increase in all dimensions of the µPBR between two-plates demonstrated very efficient and simple approach for the capacity rise. Further characterization of µPBRs of various sizes using the piezoresistive pressure sensor revealed very low pressure drops as compared to their conventional counterparts and thereby great applicability for production systems based on numbering-up approach.

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Series: Chemical engineering journal
ISSN: 1385-8947
ISSN-E: 1873-3212
ISSN-L: 1385-8947
Volume: 350
Pages: 541 - 550
DOI: 10.1016/j.cej.2018.05.028
Type of Publication: A1 Journal article – refereed
Field of Science: 215 Chemical engineering
Funding: The financial support of the EC FP7 Project EUROMBR (Grant Agreement No. 608104) and of the Ministry of Education, Science and Sport of the Republic of Slovenia through Grant P2-0191 and PhD Grant MR-39080 (FS) is acknowledged. MB was supported by the Public Scholarship, Development, Disability and Maintenance Fund of the Republic of Slovenia (Grant No. 11011-73/2013). The support of the COST Action CM1303 Systems Biocatalysis is very much appreciated.
EU Grant Number: (608104) EUROMBR - European network for innovative microbioreactor applications in bioprocess development
Copyright information: © 2018 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license