University of Oulu

Kyung Min Lee, Sami M Kivelä, Vladislav Ivanov, Axel Hausmann, Lauri Kaila, Niklas Wahlberg, Marko Mutanen; Information Dropout Patterns in Restriction Site Associated DNA Phylogenomics and a Comparison with Multilocus Sanger Data in a Species-Rich Moth Genus, Systematic Biology, Volume 67, Issue 6, 1 November 2018, Pages 925–939, https://doi.org/10.1093/sysbio/syy029

Information dropout patterns in restriction site associated DNA phylogenomics and a comparison with multilocus Sanger data in a species-rich moth genus

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Author: Lee, Kyung Min1; Kivelä, Sami M1,2; Ivanov, Vladislav1;
Organizations: 1Department of Ecology and Genetics, University of Oulu
2Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu
3SNSB – Bavarian State Collection of Zoology
4Finnish Museum of Natural History, Zoology Unit
5Department of Biology, Lund University
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2018111548211
Language: English
Published: Oxford University Press, 2018
Publish Date: 2019-04-14
Description:

Abstract

A rapid shift from traditional Sanger sequencing-based molecular methods to the phylogenomic approach with large numbers of loci is underway. Among phylogenomic methods, restriction site associated DNA (RAD) sequencing approaches have gained much attention as they enable rapid generation of up to thousands of loci randomly scattered across the genome and are suitable for nonmodel species. RAD data sets however suffer from large amounts of missing data and rapid locus dropout along with decreasing relatedness among taxa. The relationship between locus dropout and the amount of phylogenetic information retained in the data has remained largely uninvestigated. Similarly, phylogenetic hypotheses based on RAD have rarely been compared with phylogenetic hypotheses based on multilocus Sanger sequencing, even less so using exactly the same species and specimens. We compared the Sanger-based phylogenetic hypothesis (8 loci; 6172 bp) of 32 species of the diverse moth genus Eupithecia (Lepidoptera, Geometridae) to that based on double-digest RAD sequencing (3256 loci; 726,658 bp). We observed that topologies were largely congruent, with some notable exceptions that we discuss. The locus dropout effect was strong. We demonstrate that number of loci is not a precise measure of phylogenetic information since the number of single-nucleotide polymorphisms (SNPs) may remain low at very shallow phylogenetic levels despite large numbers of loci. As we hypothesize, the number of SNPs and parsimony informative SNPs (PIS) is low at shallow phylogenetic levels, peaks at intermediate levels and, thereafter, declines again at the deepest levels as a result of decay of available loci. Similarly, we demonstrate with empirical data that the locus dropout affects the type of loci retained, the loci found in many species tending to show lower interspecific distances than those shared among fewer species. We also examine the effects of the numbers of loci, SNPs, and PIS on nodal bootstrap support, but could not demonstrate with our data our expectation of a positive correlation between them. We conclude that RAD methods provide a powerful tool for phylogenomics at an intermediate phylogenetic level as indicated by its broad congruence with an eight-gene Sanger data set in a genus of moths. When assessing the quality of the data for phylogenetic inference, the focus should be on the distribution and number of SNPs and PIS rather than on loci.

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Series: Systematic biology
ISSN: 1063-5157
ISSN-E: 1076-836X
ISSN-L: 1063-5157
Volume: 67
Issue: 6
Pages: 925 - 939
DOI: 10.1093/sysbio/syy029
OADOI: https://oadoi.org/10.1093/sysbio/syy029
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
1182 Biochemistry, cell and molecular biology
Subjects:
Funding: This study was financially supported by the Academy of Finland through a research grant #277984 to MM. NW acknowledges support from the Swedish Research Council and SMK thanks Emil Aaltonen Foundation and the Estonian Research Council (grant PUT1474) for research grants.
Academy of Finland Grant Number: 277984
Detailed Information: 277984 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2018. Published by Oxford University Press, on behalf of the Society of Systematic Biologists. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in Systematic Biology following peer review. The version of record Kyung Min Lee, Sami M Kivelä, Vladislav Ivanov, Axel Hausmann, Lauri Kaila, Niklas Wahlberg, Marko Mutanen; Information Dropout Patterns in Restriction Site Associated DNA Phylogenomics and a Comparison with Multilocus Sanger Data in a Species-Rich Moth Genus, Systematic Biology, Volume 67, Issue 6, 1 November 2018, Pages 925–939, is available online at: https://doi.org/10.1093/sysbio/syy029.