University of Oulu

Hoque Apu, E., Akram, S., Rissanen, J., Wan, H., Salo, T. (2018) Desmoglein 3 – Influence on oral carcinoma cell migration and invasion. Experimental Cell Research, 370 (2), 353-364. doi:10.1016/j.yexcr.2018.06.037

Desmoglein 3 - influence on oral carcinoma cell migration and invasion

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Author: Apu, Ehsanul Hoque1,2; Akram, Saad Ullah3; Rissanen, Jouni4;
Organizations: 1Cancer and Translational Medicine Research Unit, University of Oulu
2Centre for Clinical and Diagnostic Oral Sciences, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London
3Department of Computer Science and Engineering, University of Oulu
4Fibre and Particle Engineering, University of Oulu
5Department of Oral and Maxillofacial Diseases, University of Helsinki
6Medical Research Centre, Oulu University Hospital
7HUSLAB, Department of Pathology, Helsinki University Central Hospital
8Department of Oral Diagnosis, Oral Pathology Division, Piracicaba Dental School, University of Campinas
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.7 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2018120449809
Language: English
Published: Elsevier, 2018
Publish Date: 2018-12-04
Description:

Abstract

Desmoglein 3 (Dsg3) is an adhesion receptor in desmosomes, but its role in carcinoma cell migration and invasion is mostly unknown. Our aim was to quantitatively analyse the motion of Dsg3-modified carcinoma cells in 2D settings and in 3D within tumour microenvironment mimicking (TMEM) matrices. We tested mutant constructs of C-terminally truncated Dsg3 (∆238 and ∆560), overexpressed full-length (FL) Dsg3, and empty vector control (Ct) of buccal mucosa squamous cell carcinoma (SqCC/Y1) cells. We captured live cell images and analysed migration velocities and accumulated and Euclidean distances. We compared rodent collagen and Matrigel® with human Myogel TMEM matrices for these parameters in 3D sandwich, in which we also tested the effects of monoclonal antibody AK23, which targets the EC1 domain of Dsg3. In monolayer culture, FL and both truncated constructs migrated faster and had higher accumulated distances than Ct cells. However, in the 3D assays, only the mutants invaded faster relative to Ct cells. Of the mutants, the shorter form (Δ238) exhibited faster migration and invasion than Δ560 cells. In the Transwell, all of the cells invaded faster through Myogel than Matrigel® coated wells. In 3D sandwich, AK23 antibody inhibited only the invasion of FL cells. We conclude that different experimental 2D and 3D settings can markedly influence the movement of oral carcinoma cells with various Dsg3 modifications.

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Series: Experimental cell research
ISSN: 0014-4827
ISSN-E: 1090-2422
ISSN-L: 0014-4827
Volume: 370
Issue: 2
DOI: 10.1016/j.yexcr.2018.06.037
OADOI: https://oadoi.org/10.1016/j.yexcr.2018.06.037
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Funding: The work was supported by research grants from Sigrid Juselius Foundation, Finnish Cancer Foundation, research funds from the Medical Faculty of the University of Oulu and Oulu University Hospital special state support for research and Medical Research Center Oulu, Finnish Doctoral Programme in Oral Sciences (FINDOS), Tyyni Tani Foundation, Oulu University Research Foundation, and Centre for International Mobility (CIMO)# TM-15-9587.
Copyright information: © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).