University of Oulu

HOORNSTRA, D., VESTERLIN, J., PÄRNÄNEN, P., AL-SAMADI, A., ZLOTOGORSKI-HURVITZ, A., VERED, M., SALO, T. (2018) Fermented Lingonberry Juice Inhibits Oral Tongue Squamous Cell Carcinoma Invasion In Vitro Similarly to Curcumin. In Vivo, 32 (5), 1089-1095. doi:10.21873/invivo.11350

Fermented lingonberry juice inhibits oral tongue squamous cell carcinoma invasion in vitro similarly to curcumin

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Author: Hoornstra, Douwe1; Vesterlin, Jenni1; Pärnänen, Pirjo1;
Organizations: 1Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital
2Department of Oral Pathology and Oral Medicine, School of Dental Medicine, Tel Aviv University
3Institute of Pathology, The Chaim Sheba Medical Center
4Cancer and Translational Medicine Research Unit, University of Oulu
5Medical Research Center, Oulu University Hospital
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
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Language: English
Published: International Institute of Anticancer Research, 2018
Publish Date: 2018-12-07


Background: Oral tongue squamous cell carcinoma (OTSCC) cells are highly proliferative and invasive. Lingonberry contains several polyphenolic compounds similar to curcumin. We hypothesize that fermented lingonberry juice (FLJ) has an anti-invasive and anti-proliferative effect on OTSCC cells similarly to curcumin, which is known to be anti-carcinogenic.

Materials and methods: FLJ, curcumin dissolved in ethanol, or curcumin loaded in Candida extracellular vesicles (EVs) were added to more (HSC-3) and less aggressive (SCC-25) OTSCC cells. Cell proliferation was measured with a 5-bromo-2’-deoxyuridine kit and invasion in the three-dimensional Myogel spheroid assay. Statistical analyses were completed with one-way ANOVA and Bonferroni post-hoc testing.

Results: Both FLJ and curcumin significantly reduced the proliferation and invasion of HSC-3 and SCC-25 cells. The effects of curcumin were not improved when cells were treated with curcumin loaded within EVs.

Conclusions: Our results suggest that FLJ, like curcumin, has an anti-carcinogenic effect on aggressive OTSCC cells in vitro.

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Series: In vivo
ISSN: 0258-851X
ISSN-E: 1791-7549
ISSN-L: 0258-851X
Volume: 32
Issue: 5
Pages: 1089 - 1095
DOI: 10.21873/invivo.11350
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: This work was supported by the Sigrid Juselius Foundation, the Cancer Society of Finland, and Helsinki University Central Hospital.
Copyright information: Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. Published in this repository with the kind permission of the publisher.