University of Oulu

Raspini, G., Wolff, J., Helminen, M., Raspini, G., Raspini, M., Sándor, G. (2018) Dental Stem Cells Harvested from Third Molars Combined with Bioactive Glass Can Induce Signs of Bone Formation In Vitro. Journal of Oral Maxillofacial Research, 9 (1), e2. doi:10.5037/jomr.2018.9102

Dental stem cells harvested from third molars combined with bioactive glass can induce signs of bone formation in vitro

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Author: Raspini, Gregorio1; Wolff, Jan2; Helminen, Mika3;
Organizations: 1Department of Oral and Maxillofacial Surgery, University of Oulu
2Department of Oral and Maxillofacial Surgery/Pathology & 3D Innovation Lab, VU University Medical Center
3Faculty of Social Sciences, University of Tampere and Science Center, Pirkanmaa Hospital District
4Private practice, Arezzo, Italy
5Tissue Engineering and Oral and Maxillofacial Surgery, Medical Research Center, University of Oulu and Oulu University Hospital
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.8 MB)
Persistent link:
Language: English
Published: Stilus Optimus, 2018
Publish Date: 2019-01-08


Objectives: The aim of this study was to assess the interaction of a bioactive glass scaffold with cells derived from dental pulp, dental follicle and periodontal ligament.

Material and methods: Impacted third molars were surgically removed from three young donors. Cells from the dental pulp, follicle and periodontal ligament tissues were isolated and expanded. Different cell populations were characterised using specific CD markers. Expanded pulp, follicle and periodontal cells were then seeded onto bioactive glass scaffolds and cultured in osteogenic medium or basic medium. Cell attachment, viability, proliferation and alkaline phosphatase activity were assessed.

Results: This study revealed good biocompatibility of the specific bioactive glass configuration tested and the osteogenic induction of cells derived from dental pulp, dental follicle and periodontal ligament. Osteogenic medium seemed to increase the differentiation pattern and dental pulp stem cells showed the most positive results compared to periodontal ligament and dental follicle stem cells.

Conclusions: Dental pulp stem cells combined with a bioactive glass scaffold and exposed to osteogenic medium in vitro represent a promising combination for future study of hard tissue regeneration in the cranio-maxillofacial skeleton.

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Series: Journal of oral & maxillofacial research
ISSN: 2029-283X
ISSN-E: 2029-283X
ISSN-L: 2029-283X
Volume: 9
Issue: 1
Article number: e2
DOI: 10.5037/jomr.2018.9102
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
313 Dentistry
Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Copyright information: © Raspini G, Wolff J, Helminen M, Raspini G, Raspini M, Sándor GK. Published in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH (, 31 March 2018. This is an open-access article, first published in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH, distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work and is properly cited. The copyright, license information and link to the original publication on ( must be included.