University of Oulu

Stape, T., Seseogullari-Dirihan, R., Tjäderhane, L., Abuna, G., Martins, L., Tezvergil-Mutluay, A. (2018) A novel dry-bonding approach to reduce collagen degradation and optimize resin-dentin interfaces. Scientific Reports, 8 (1), 16890. doi:10.1038/s41598-018-34726-8

A novel dry-bonding approach to reduce collagen degradation and optimize resin-dentin interfaces

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Author: Stape, Thiago Henrique Scarabello1,2; Seseogullari-Dirihan, Roda1,2; Tjäderhane, Leo3,4;
Organizations: 1Department of Restorative Dentistry and Cariology, Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku
2Turku University Hospital, TYKS, University of Turku
3Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland and Helsinki University Hospital
4Research Unit of Oral Health Sciences, Medical Research Center Oulu (MRC Oulu), Oulu University Hospital and University of Oulu
5Piracicaba Dental School, University of Campinas, Department of Restorative Dentistry, Dental Materials Area
6Piracicaba Dental School, University of Campinas, Department of Restorative Dentistry
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201901182497
Language: English
Published: Springer Nature, 2018
Publish Date: 2019-01-18
Description:

Abstract

In dentistry, the wet-bonding approach relies on water to maintain demineralized collagen expanded for proper resin infiltration; nevertheless, hydrolytic instability of the resin-dentin interface is inevitable with current bonding techniques. Considering dimethyl sulfoxide’s (DMSO) ability to “biomodify” collagen and precipitate enzymes, the aim was to test whether the use of DMSO would permit adequate resin bonding to H3PO4-etched dehydrated dentin and assess its impact on collagen degradation by host-derived enzymes. Etched dentin surfaces from extracted sound human molars were randomly bonded in wet or dry conditions using aqueous or ethanolic DMSO solutions as pretreatments and bonding resins with or without DMSO. Bonded teeth were sectioned into resin-dentin slabs for confocal in situ zymography and beams for microtensile bond strength test. Demineralized powdered dentin was incubated in the tested DMSO -media and a hydroxyproline assay evaluated dissolution of collagen peptides. Zymography was performed on protein extracts obtained from dry and wet H3PO4-ecthed dentin powder treated with the DMSO- media. The correlative biochemical analysis demonstrated that reduction of water content during dentin hybridization by the innovative dry-bonding approaches with DMSO is effective to inactivate host-derived MMP-2 and MMP-9 and thus reduce collagen degradation while simultaneously optimizing resin-dentin bonding.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 8
Article number: 16890
DOI: 10.1038/s41598-018-34726-8
OADOI: https://oadoi.org/10.1038/s41598-018-34726-8
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Subjects:
Funding: This work was supported by grant #296653 from the Academy of Finland to AT-M (PI), EVO funding of Turku University Hospital to AT-M (PI) and CIMO fellowship funding to THSS.
Academy of Finland Grant Number: 296653
Detailed Information: 296653 (Academy of Finland Funding decision)
Dataset Reference: Electronic supplementary material:
  https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-018-34726-8/MediaObjects/41598_2018_34726_MOESM1_ESM.pdf
Copyright information: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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