University of Oulu

Sdobnov, AY, Darvin, ME, Schleusener, J, Lademann, J, Tuchin, VV. Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy. J. Biophotonics. 2019; 12:e201800283. https://doi.org/10.1002/jbio.201800283

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents : quantitative analysis using confocal Raman microscopy

Saved in:
Author: Sdobnov, Anton Y.1,2; Darvin, Maxim E.3; Schleusener, Johannes3;
Organizations: 1Faculty of Information Technology and Electrical Engineering, University of Oulu, Oulu, Finland
2Department of Optics and Biophotonics, Research‐Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, Russia
3Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt‐Universität zu Berlin, Berlin Institute of Health, Berlin, Germany
4Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of RAS, Saratov, Russia
5Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Tomsk, Russia
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe201901242876
Language: English
Published: John Wiley & Sons, 2019
Publish Date: 2019-12-18
Description:

Abstract

Confocal Raman microscopy has been used to measure depth‐dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque [300]) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 minutes. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 minutes), 33.1% for glycerol (30 minutes), 8.3% for Omnipaque (60 minutes) and 4.4% for Omnipaque (30 minutes), on average for the 40 to 200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5% and 4.6%), strongly bound (50.4%, 33.0%, 7.9% and 3.4%), tightly bound (63.6%, 42.3%, 26.1% and 12.9%) and unbound (55.4%, 28.7%, 10.1% and 5.9%) water types on average for the 40 to 200 μm depths, post application of glycerol (60 minutes), glycerol (30 minutes), Omnipaque (60 minutes) and Omnipaque (30 minutes), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA‐induced water flux in the skin, and thus, are responsible for optical clearing efficiency.

see all

Series: Journal of biophotonics
ISSN: 1864-063X
ISSN-E: 1864-0648
ISSN-L: 1864-063X
Volume: 12
Issue: 5
Article number: e201800283
DOI: 10.1002/jbio.201800283
OADOI: https://oadoi.org/10.1002/jbio.201800283
Type of Publication: A1 Journal article – refereed
Field of Science: 3141 Health care science
Subjects:
Funding: The study was supported by the German Foundation Centre Essen, Foundation for Skin Physiology (T335). A.Y.S. is thankful to the Research Program of Saratov State University for financial support of visiting Charité–Universitätsmedizin Berlin. V.V.T. was supported by the Russian Federation MES grant 17.1223.2017/AP and the RF Governmental grant 14.W03.31.0023.
Copyright information: © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Sdobnov AY, Darvin ME, Schleusener J, Lademann J, Tuchin VV. Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy. J. Biophotonics. 2019;e201800283, which has been published in final form at https://doi.org/10.1002/jbio.201800283. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.