University of Oulu

Elena K. Volkova, Irina Yu Yanina, Elina A. Genina, Alexey N. Bashkatov, Julia G. Konyukhova, Alexey P. Popov, Elena S. Speranskaya, Alla B. Bucharskaya, Nikita A. Navolokin, Irina Yu. Goryacheva, Vyacheslav I. Kochubey, Gleb B. Sukhorukov, Igor V. Meglinski, Valery V. Tuchin, “Delivery and reveal of localization of upconversion luminescent microparticles and quantum dots in the skin in vivo by fractional laser microablation, multimodal imaging, and optical clearing,” J. Biomed. Opt. 23(2), 026001 (2018), doi: 10.1117/1.JBO.23.2.026001

Delivery and reveal of localization of upconversion luminescent microparticles and quantum dots in the skin in vivo by fractional laser microablation, multimodal imaging, and optical clearing

Saved in:
Author: Volkova, Elena1,2,3; Yanina, Irina2,3; Genina, Elina2,3;
Organizations: 1University of Oulu, Optoelectronics and Measurement Techniques Research Unit, Oulu, Finland
2Saratov State University (National Research University), Optics and Biophotonics Department, Saratov, Russia
3Tomsk State University (National Research University), Interdisciplinary Laboratory of Biophotonics, Tomsk, Russia
4ITMO University, Terahertz Biomedicine Laboratory, St. Petersburg, Russia
5Saratov State University (National Research University), General and Inorganic Chemistry Department, Saratov, Russia
6Saratov State Medical University, Saratov, Russia
7Queen Mary University of London, School of Engineering and Materials Science, London, United Kingdom
8Irkutsk State University, Institute of Biology, Irkutsk, Russia
9ITMO University, Laboratory of Femtomedicine, St. Petersburg, Russia
10Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Laboratory of Laser Diagnostics of Technical and Living Systems, Saratov, Russia
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201902084432
Language: English
Published: International Society for Optical Engineering, 2018
Publish Date: 2019-02-08
Description:

Abstract

Delivery and spatial localization of upconversion luminescent microparticles [Y2O3:Yb, Er] (mean size ∼1.6  μm) and quantum dots (QDs) (CuInS2/ZnS nanoparticles coated with polyethylene glycol-based amphiphilic polymer, mean size ∼20  nm) inside rat skin was studied in vivo using a multimodal optical imaging approach. The particles were embedded into the skin dermis to the depth from 300 to 500  μm through microchannels performed by fractional laser microablation. Low-frequency ultrasound was applied to enhance penetration of the particles into the skin. Visualization of the particles was revealed using a combination of luminescent spectroscopy, optical coherence tomography, confocal microscopy, and histochemical analysis. Optical clearing was used to enhance the image contrast of the luminescent signal from the particles. It was demonstrated that the penetration depth of particles depends on their size, resulting in a different detection time interval (days) of the luminescent signal from microparticles and QDs inside the rat skin in vivo. We show that luminescent signal from the upconversion microparticles and QDs was detected after the particle delivery into the rat skin in vivo during eighth and fourth days, respectively. We hypothesize that the upconversion microparticles have created a long-time depot localized in the laser-created channels, as the QDs spread over the surrounding tissues.

see all

Series: Journal of biomedical optics
ISSN: 1083-3668
ISSN-E: 1560-2281
ISSN-L: 1083-3668
Volume: 23
Issue: 2
Article number: 026001
DOI: 10.1117/1.JBO.23.2.026001
OADOI: https://oadoi.org/10.1117/1.JBO.23.2.026001
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
221 Nanotechnology
3126 Surgery, anesthesiology, intensive care, radiology
Subjects:
Funding: This study was supported by Academy of Finland (288164 EKV, IYuY; 260321, 290596, APP); E.K.V. was also supported by the Russian Governmental contract 2014/203, Project 1490; E.A.G., A.B.B., N.A.N., G.B.S., and V.V.T. were supported by the Russian Governmental grant 14. Z50.31.0004; I. Yu Y. and E.K.V. were supported by the Russian Presidential grant MK-6009.2016.2; E.K.V., I. Yu Y., A.N.B., V.I.K., A.P.P., I.V.M., and V.V.T. acknowledge support by the Russian Governmental grant 074-U01; E.K.V., I. Yu Y., E.A.G., A.N.B., J.G.K., V.I.K., and V.V.T. were supported by the Russian Presidential grant NSh-7898.2016.2.
Academy of Finland Grant Number: 288164
260321
290596
Detailed Information: 288164 (Academy of Finland Funding decision)
260321 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
Copyright information: © 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).