University of Oulu

Kögler M, Ryabchikov YV, Uusitalo S, et al. Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification. J. Biophotonics. 2018;11:e201700225. https://doi.org/10.1002/jbio.201700225

Bare laser-synthesized Au-based nanoparticles as nondisturbing surface-enhanced Raman scattering probes for bacteria identification

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Author: Kögler, Martin1,2; Ryabchikov, Yury3,4; Uusitalo, Sanna5;
Organizations: 1Centre for Drug Research, Division of Pharmaceutical Biosciences, Faculty of Pharmacy of the University of Helsinki, Viikinkaari 5E, FI-00014 Helsinki, Finland
2Chair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 71-76, D-13355 Berlin, Germany
3Aix-Marseille University, CNRS, LP3 UMR 7341, Campus de Luminy – Case 917, 13288, Marseille Cedex 9, France
4P.N. Lebedev Physical Institute of Russian Academy of Sciences, 53 Leninskii Prospekt, Moscow 199 991, Russia
5VTT - Technical Research Centre of Finland, Kaitoväylä 1, 90590 Oulu, Finland
6Optoelectronics and Measurement Techniques, Faculty of Information Technology and Electrical Engineering, University of Oulu, Erkki Koiso-Kanttilan katu 3, FI-90570 Oulu, Finland
7ITMO University, 49 Kronverksky Prospekt, St. Petersburg, 197101 Russia
8National Resources Institute Finland (LUKE) Bio-based business and industry, University of Oulu, Paavo Havaksen tie 3, FI-90014 Oulu, Finland
9Natural Research Institute Finland (LUKE), Bio-based business and industry, Itäinen pitkäkatu 3,20520 Turku
10National Research Nuclear University “MEPhI”, Institute of Engineering Physics for Biomedicine (PhysBio), Bio-Nanophotonic Lab., 115409 Moscow, Russia
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.8 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe201902114499
Language: English
Published: John Wiley & Sons, 2018
Publish Date: 2019-02-01
Description:

Abstract

The ability of noble metal‐based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface‐enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by‐products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser‐synthesized Au‐based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au‐based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser‐synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination‐free laser‐synthesized nanomaterials.

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Series: Journal of biophotonics
ISSN: 1864-063X
ISSN-E: 1864-0648
ISSN-L: 1864-063X
Volume: 11
Issue: 7
Article number: UNSP e201700225
DOI: 10.1002/jbio.201700225
OADOI: https://oadoi.org/10.1002/jbio.201700225
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
217 Medical engineering
Subjects:
Funding: M. Kögler acknowledges the support by Academy of Finland through FOULSENS project (292253). Yu.V. Ryabchikov acknowledges a support from COST project (ECOST-STSM-BM1205-120416-072252) and from Center for Research Strategy of Free University of Berlin (0503121810) for performing experiments. S. Uusitalo acknowledges the financial support by Academy of Finland through M-SPEC project (284907). A. Popov acknowledges Academy of Finland (projects 260321, 290596). This work was also partially supported by Government of Russian Federation, Grant 074-U01. Y. Ryabchikov, A. Popov, G. Tselikov, A. V. Kabashin acknowledge contributions from “LASERNANOCANCER” (No. PC201420) and GRAVITY projects of the ITMO “Plan Cancer 2014–2019” INSERM program.
Academy of Finland Grant Number: 260321
290596
Detailed Information: 260321 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
Copyright information: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Kögler M, Ryabchikov YV, Uusitalo S, et al. Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification. J. Biophotonics. 2018;11:e201700225. https://doi.org/10.1002/jbio.201700225, which has been published in final form at 10.1002/jbio.201700225. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.