University of Oulu

Tatiana Avsievich, Alexey Popov, Alexander Bykov, and Igor Meglinski, "Mutual interaction of red blood cells assessed by optical tweezers and scanning electron microscopy imaging," Opt. Lett. 43, 3921-3924 (2018). 10.1364/OL.43.003921

Mutual interaction of red blood cells assessed by optical tweezers and scanning electron microscopy imaging

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Author: Avsievich, Tatiana1; Popov, Alexey1; Bykov, Alexander1;
Organizations: 1Opto-Electronic and Measurement Techniques, University of Oulu, P.O. Box 4500, Oulu, 90014, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
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Language: English
Published: Optical Society of America, 2018
Publish Date: 2019-08-08


The adhesion of red blood cells (RBC) has been studied extensively in frame of cell-to-cell interaction induced by dextran macromolecules, whereas the data are lacking for native plasma solution. We apply optical tweezers to investigate the induced adhesion of RBC in plasma and in dextran solution. Two hypotheses, cross-bridges and depletion layer, are typically used to describe the mechanism of cell interaction; however, both mechanisms need to be confirmed experimentally. These interactions in fact are very much dependent on the size and concentration of dextran and proteins in plasma. The results show that in different dextran solutions, the interaction of adhering RBC agrees well with the quantitative predictions obtained based on the depletion-induced cells adhesion model, whereas the migrating cross-bridges model is more appropriate for plasma. Despite the different mechanisms of RBC interaction in a mixture of dextran with the size ranges and volume fraction proportional to plasma proteins, the dependence of RBC adhering tends to be close to the cross-bridges model. The induced aggregation of RBC in the dextran solutions and in native plasma are observed by direct visualization utilizing scanning electron microscopy.

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Series: Optics letters
ISSN: 0146-9592
ISSN-E: 1539-4794
ISSN-L: 0146-9592
Volume: 43
Issue: 16
Pages: 3921 - 3924
DOI: 10.1364/OL.43.003921
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Funding: Authors acknowledge CIMO Fellowship (grant TM-17-10370) and financial support from Academy of Finland (projects 314369, 290596, 311698). Authors also acknowledge initial involvement of Dr. K. Lee and Prof. A.V. Priezzhev from M.V. Lomonosov Moscow State University (Russia) and Dr. A. Karmenyan from Physics Department of National Don Hwa University (Hualien, Taiwan) at the early stage of the OT development.
Academy of Finland Grant Number: 314369
Detailed Information: 314369 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
311698 (Academy of Finland Funding decision)
Copyright information: © 2018 Optical Society of America.