University of Oulu

Tommi Palomäki, Emilia Peltola, Sami Sainio, Niklas Wester, Olli Pitkänen, Krisztian Kordas, Jari Koskinen, Tomi Laurila, Unmodified and multi-walled carbon nanotube modified tetrahedral amorphous carbon (ta-C) films as in vivo sensor materials for sensitive and selective detection of dopamine, Biosensors and Bioelectronics, Volume 118, 2018, Pages 23-30, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2018.07.018.

Unmodified and multi-walled carbon nanotube modified tetrahedral amorphous carbon (ta-C) films as in vivo sensor materials for sensitive and selective detection of dopamine

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Author: Palomäki, Tommi1; Peltola, Emilia1; Sainio, Sami2;
Organizations: 1Department of Electrical Engineering and Automation, School of Electrical Engineering, Aalto University
2Department of Chemistry and Materials Science, School of Chemical Technology, Aalto University
3Microelectronics Research Unit, Faculty of Information Technology and Electrical Engineering, University of Oulu
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe201903148891
Language: English
Published: Elsevier, 2018
Publish Date: 2020-07-11
Description:

Abstract

Unmodified and multi-walled carbon nanotube (MWCNT) modified tetrahedral amorphous carbon (ta-C) films of 15 and 50 nm were investigated as potential in vivo sensor materials for the detection of dopamine (DA) in the presence of the main interferents, ascorbic acid (AA) and uric acid (UA). The MWCNTs were grown directly on ta-C by chemical vapor deposition (designated as ta-C+CNT) and were characterized with X-ray photoelectron spectroscopy, Raman spectroscopy, scanning and transmission electron microscopy. Electroanalytical sensitivity and selectivity were determined with cyclic voltammetry. Biocompatibility of the materials was assessed with cell cultures of mouse neural stem cells (mNSCs). The detection limits of DA for both ta-C and ta-C+CNT electrodes ranged from 40 to 85 nM, which are well within the required range for in vivo detection. The detection limits were lower for both ta-C and ta-C+CNT electrodes with 50 nm of ta-C compared to 15 nm. The ta-C electrodes showed a large dynamic linear range of 0.01–100 µM but could not resolve between the oxidation peaks of DA, AA and UA. Modification with MWCNTs, however, resulted in excellent selectivity and all three analytes could be detected simultaneously at physiologically relevant concentrations using cyclic voltammetry. Based on cell culture of mNSCs, both ta-C and ta-C+CNT exhibited good biocompatibility, demonstrating their potential as in vivo sensor materials for the detection of DA.

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Series: Biosensors & bioelectronics
ISSN: 0956-5663
ISSN-E: 1873-4235
ISSN-L: 0956-5663
Volume: 118
Pages: 23 - 30
DOI: 10.1016/j.bios.2018.07.018
OADOI: https://oadoi.org/10.1016/j.bios.2018.07.018
Type of Publication: A1 Journal article – refereed
Field of Science: 221 Nanotechnology
216 Materials engineering
217 Medical engineering
Subjects:
Funding: This work was supported by the Academy of Finland (E.P. grant #274670, T.P. and T.L. grant #285526).
Dataset Reference: Supplementary material:
  https://ars.els-cdn.com/content/image/1-s2.0-S0956566318305190-mmc1.docx
https://www.sciencedirect.com/topics/engineering/supplementary-material
Copyright information: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
  https://creativecommons.org/licenses/by-nc-nd/4.0/