University of Oulu

Sanni M. E. Kinnunen, Satu K. Mänttäri, and Seppo Y. O. Saarela, "Expression of AMPK, SIRT1, and ACC Differs between Winter- and Summer-Acclimatized Djungarian Hamsters," Physiological and Biochemical Zoology 90, no. 6 (November/December 2017): 605-612. https://doi.org/10.1086/694295. PMID: 28880122

Expression of AMPK, SIRT1, and ACC differs between winter- and summer-acclimatized Djungarian hamsters

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Author: Kinnunen, Sanni M. E.1; Mänttäri, Satu K.2; Saarela, Seppo Y. O.1
Organizations: 1Department of Genetics and Physiology, University of Oulu, PO Box 3000, FI-90014 Oulu, Finland
2Finnish Institute of Occupational Health, Aapistie 1, FI-90220 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019032610003
Language: English
Published: University of Chicago Press, 2017
Publish Date: 2019-03-26
Description:

Abstract

The wintering strategy of the Djungarian hamster (Phodopus sungorus) includes a naturally occurring decrease in food intake and body mass. Our aim was to investigate the conceivable role of the metabolic regulators, AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1), in the seasonal adaptation of the Djungarian hamster. In addition, a rate-limiting enzyme in fatty acid synthesis and oxidation, acetyl CoA carboxylase (ACC), was studied. Relative protein expressions and phosphorylated forms (pAMPK and pACC) were determined by Western blot from subcutaneous white adipose tissues (sWAT), abdominal white adipose tissues (aWAT), interscapular brown adipose tissues (iBAT), skeletal muscle, and hypothalamus of winter- and summer-acclimatized hamsters. The winter group had higher AMPK expression in sWAT, aWAT, and iBAT, but the relative amount of phosphorylated protein (pAMPK/AMPK ratio) was lower in these tissues. Furthermore, ACC expression was higher in sWAT and iBAT of the winter animals. pACC (inactive form) levels were higher in all adipose tissues, yet a lower pACC/ACC ratio was detected in iBAT of the winter hamsters. Muscle AMPK expression was lower but pAMPK/AMPK ratio higher in the winter group. SIRT1 expression was higher in muscle and all adipose tissues of the winter hamsters. Hypothalamic protein expressions did not differ between the groups. Higher expressions of AMPK, ACC, and SIRT1 in WAT and iBAT of the winter hamsters suggest a role in the regulation of lipid reserves and increased thermogenic capacity characteristic to the winter-adapted Djungarian hamsters.

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Series: Physiological and biochemical zoology
ISSN: 1522-2152
ISSN-E: 1537-5293
ISSN-L: 1522-2152
Volume: 90
Issue: 6
Pages: 605 - 612
DOI: 10.1086/694295
OADOI: https://oadoi.org/10.1086/694295
Type of Publication: A1 Journal article – refereed
Field of Science: 1184 Genetics, developmental biology, physiology
413 Veterinary science
Subjects:
Funding: This study was financially supported by the Academy of Finland (grant 134048 to S.S.) and by the Emil Aaltonen Foundation (personal working grant to S.M.E.K).
Academy of Finland Grant Number: 134048
Detailed Information: 134048 (Academy of Finland Funding decision)
Copyright information: © 2017 by The University of Chicago. All rights reserved.