University of Oulu

Hämälä, T, Mattila, TM, Leinonen, PH, Kuittinen, H, Savolainen, O. Role of seed germination in adaptation and reproductive isolation in Arabidopsis lyrata. Mol Ecol. 2017; 26: 3484– 3496. https://doi.org/10.1111/mec.14135

Role of seed germination in adaptation and reproductive isolation in Arabidopsis lyrata

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Author: Hämälä, Tuomas1,2; Mattila, Tiina M.1; Leinonen, Päivi H.1;
Organizations: 1Department of Ecology and Genetics, University of Oulu, Oulu, Finland
2Biocenter Oulu, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019040411075
Language: English
Published: John Wiley & Sons, 2017
Publish Date: 2019-04-04
Description:

Abstract

Seed germination is an important developmental and life history stage. Yet, the evolutionary impact of germination has mainly been studied in the context of dormancy, or for its role in reproductive isolation between species. Here, we aim to examine multiple consequences of genetic divergence on germination traits between two Arabidopsis lyrata subspecies: ssp. petraea (Eurasia) and ssp. lyrata (North America). Postdormancy germination time, a potentially adaptive trait, showed differentiation between the populations, and quantitative trait loci (QTL) mapping revealed that the trait variation is mainly controlled by two antagonistic loci. These QTL areas contain several candidate genes with known function in postdormancy germination in A. thaliana. The sequence variation of three genes was consistent with differential selection, and they also included fixed nonsynonymous substitutions with potential to account for the phenotypic differentiation. We further show that the divergence between the subspecies has led to a slight but significant reduction in hybrid germination proportions, indicating incipient reproductive isolation. Comparison of reciprocal F1 and F2 progenies suggests that Bateson–Dobzhansky–Muller incompatibilities likely act through uniparentally inherited factors. Examination of genomewide transmission ratio distortion further revealed that cytonuclear interactions cause substantial pregermination inviability in the hybrids. These results confirm that seed germination has adaptive potential beyond the dormancy stage and that hybrid seed inviability can be one of the first reproductive barriers to arise during divergence.

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Series: Molecular ecology
ISSN: 0962-1083
ISSN-E: 1365-294X
ISSN-L: 0962-1083
Volume: 26
Issue: 13
Pages: 3484 - 3496
DOI: 10.1111/mec.14135
OADOI: https://oadoi.org/10.1111/mec.14135
Type of Publication: A1 Journal article – refereed
Field of Science: 1184 Genetics, developmental biology, physiology
1181 Ecology, evolutionary biology
Subjects:
Funding: This work was supported by Biocenter Oulu and Academy of Finland's Research Council for Biosciences and Environment (grant numbers: 136804 and 132611).
Academy of Finland Grant Number: 136804
132611
Detailed Information: 136804 (Academy of Finland Funding decision)
132611 (Academy of Finland Funding decision)
Copyright information: © 2017 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Hämälä, T, Mattila, TM, Leinonen, PH, Kuittinen, H, Savolainen, O. Role of seed germination in adaptation and reproductive isolation in Arabidopsis lyrata. Mol Ecol. 2017; 26: 3484– 3496, which has been published in final form at https://doi.org/10.1111/mec.14135. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.