University of Oulu

Janis Spigulis, Ilze Oshina, Anna Berzina, Alexander Bykov, "Smartphone snapshot mapping of skin chromophores under triple-wavelength laser illumination," J. Biomed. Opt. 22(9) 091508 (2 March 2017) doi: 10.1117/1.JBO.22.9.091508

Smartphone snapshot mapping of skin chromophores under triple-wavelength laser illumination

Saved in:
Author: Spigulis, Janis1; Oshina, Ilze1; Berzina, Anna1;
Organizations: 1University of Latvia, Biophotonics Laboratory, Institute of Atomic Physics and Spectroscopy, Riga, Latvia
2University of Oulu, Optoelectronics and Measurement Techniques Unit, Faculty of Information Technology and Electrical Engineering, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 9.6 MB)
Persistent link:
Language: English
Published: SPIE, 2017
Publish Date: 2019-04-15


Chromophore distribution maps are useful tools for skin malformation severity assessment and for monitoring of skin recovery after burns, surgeries, and other interactions. The chromophore maps can be obtained by processing several spectral images of skin, e.g., captured by hyperspectral or multispectral cameras during seconds or even minutes. To avoid motion artifacts and simplify the procedure, a single-snapshot technique for mapping melanin, oxyhemoglobin, and deoxyhemoglobin of in-vivo skin by a smartphone under simultaneous three-wavelength (448–532–659 nm) laser illumination is proposed and examined. Three monochromatic spectral images related to the illumination wavelengths were extracted from the smartphone camera RGB image data set with respect to crosstalk between the RGB detection bands. Spectral images were further processed accordingly to Beer’s law in a three chromophore approximation. Photon absorption path lengths in skin at the exploited wavelengths were estimated by means of Monte Carlo simulations. The technique was validated clinically on three kinds of skin lesions: nevi, hemangiomas, and seborrheic keratosis. Design of the developed add-on laser illumination system, image-processing details, and the results of clinical measurements are presented and discussed.

see all

Series: Journal of biomedical optics
ISSN: 1083-3668
ISSN-E: 1560-2281
ISSN-L: 1083-3668
Volume: 22
Issue: 9
Article number: 091508
DOI: 10.1117/1.JBO.22.9.091508
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
214 Mechanical engineering
Funding: This study was supported by the Latvian National Research Program SOPHIS under the Grant agreement #10-4/VPP-4/11. Kind assistance of Dr. Aivars Vembris (ISSP, UL) in production of the light collecting/diffusing element is highly appreciated. AB acknowledges the support provided by Academy of Finland (Grant Nos. 296408 and 290596) and CSC–IT Center for Science, Finland, for computational resources. The fruitful collaboration between groups of two universities was facilitated by the European Commission COST action BM1205.
Academy of Finland Grant Number: 296408
Detailed Information: 296408 (Academy of Finland Funding decision)
290596 (Academy of Finland Funding decision)
Copyright information: © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE [DOI:10.1117/1.JBO.22.9.091508].