University of Oulu

Bibikova, O., Singh, P., Popov, A., Akchurin, G., Skaptsov, A., Skovorodkin, I., … Tuchin, V. (2017). Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. Laser Physics Letters, 14(5), 55901.

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

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Author: Bibikova, O.1,2,3,4; Singh, P.5; Popov, A.1,6,7;
Organizations: 1University of Oulu, Faculty of Information Technology and Electrical Engineering, Erkki Koiso-Kanttilan katu 3, Oulu, Finland, 90570
2Research-Education Institute of Optics and Biophotonics, Saratov National Research State University, 83 Astrakhanskaya street, Saratov 410012, Russian Federation
3Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Faculty of Natural Sciences, Department of Chemistry, 11Albert-Einstein-Allee, Ulm 89081, Germany
4art photonics GmbH, Rudower Chausee 46, Berlin, Germany
5Laboratory of Developmental Biology, Center for Cell-Matrix Research, Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, Faculty of Medicine, 5A Aapistie, Oulu 90220, Finland
6Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 36 Prospect Lenina, Tomsk 634050, Russian Federation
7ITMO University, 49 Kronverkskiy Prospect, St Petersburg 197101, Russian Federation
8Institute of Precision Mechanics and Control, Russian Academy of Sciences, 24 Rabochaya street, Saratov 410028, Russian Federation
9Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospect Entuziastov, Saratov 410049, Russian Federation
10SaratovLaser LLC, 56-5а Volskaya street, Saratov 410072, Russian Federation
11Faculty of Nano- and Biomedical Technologies, Saratov National Research State University, 83Astrakhanskaya street, Saratov 410012, Russian Federation
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
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Language: English
Published: IOP Publishing, 2017
Publish Date: 2019-05-02


Laser optoporation of cells by local heating of plasmonic gold nanoparticles (GNPs) was proven as a favorable delivery method of molecules into cells. The optoporation efficiency depends on the laser beam intensity and GNP properties. Here, we evaluate the membrane optoporation in vitro in terms of fluorescent dye permeability under treatment of a multi-pulsed nanosecond 1064 nm laser with a sharply-focused beam. Anisotropic GNPs, such as nanorods and nanostars, were fabricated to achieve the optimal GNP-cell interaction. Nanostars demonstrated highest optoporation efficacy with more than 80% of permeabilized cells within the illuminated area. By contrast to common laser techniques, the laser beam scanning method results in cell optoporation within a controllable programmed in advance irradiated area.

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Series: Laser physics letters
ISSN: 1612-2011
ISSN-E: 1612-202X
ISSN-L: 1612-2011
Volume: 14
Issue: 5
Article number: 055901
DOI: 10.1088/1612-202X/aa63ae
Type of Publication: A1 Journal article – refereed
Field of Science: 221 Nanotechnology
114 Physical sciences
Funding: This study was supported by the Infotech Oulu Graduate School and the Opto-Electronics and Measurement Techniques Research Unit of the University of Oulu, Finland; the Russian Science Foundation, project no. 14-15-00186 and no. 14-13-01167 (the work by VK, VB, and NK). Authorsgreatly appreciate the members of Center of Microscopy and Nanotechnology for the guidance and help. Authors are thankful to Risto Myllyla for his support and encouragement.
Copyright information: © 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at