Talaikova, N. A., Popov, A. P., Kalyanov, A. L., Ryabukho, V. P., & Meglinski, I. V. (2017). Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells. Laser Physics Letters, 14(10), 105601. https://doi.org/10.1088/1612-202x/aa7d38
Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells
|Author:||Talaikova, N.A.1,2; Popov, A.P.1,3,4; Kalyanov, A.L.5;|
1Optoelectronics and Measurement Techniques Research Unit, University of Oulu, P.O. Box 4500, Oulu, FI-90014 Finland
2Physics Faculty, Saratov State University, Astrachanskaya str., 83, Saratov, 410012, Russia
3Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, 634050, Russia
4ITMO University, Kronverkskiy Prospect 49, St Petersburg 197101, Russia
5Clinic of Neonatology, University Hospital Zurich, Zurich, CH-8091, Switzerland
6Institute of Precision Mechanics and Control, Russian Academy of Sciences, Rabochaya str., 24, Saratov, 410028, Russia
7Institute of Biology, Irkutsk State University, Irkutsk, 66400, Russia
|Online Access:||PDF Full Text (PDF, 0.8 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019050214019
|Publish Date:|| 2019-05-02
A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.
Laser physics letters
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
114 Physical sciences
217 Medical engineering
Authors acknowledge the support provided by CIMO Foundation (CIMO Fellowship, № TM-15-9625), as well as partial support provided by The Ministry of Science and Education of Russian Federation, №3.1340.2014/K., grant project “Development of optical methods and instrumentation for measurements and control of structure and dynamics of biological media”, government contract №2014/203 and by Government of Russian Federation, Grant 074-U01. Professor Meglinski also acknowledges partial support provided by Russian Science Foundation project №15-14-10008.
© 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/1612-202X/aa7d38.