University of Oulu

Talaikova, N. A., Popov, A. P., Kalyanov, A. L., Ryabukho, V. P., & Meglinski, I. V. (2017). Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells. Laser Physics Letters, 14(10), 105601.

Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

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Author: Talaikova, N.A.1,2; Popov, A.P.1,3,4; Kalyanov, A.L.5;
Organizations: 1Optoelectronics and Measurement Techniques Research Unit, University of Oulu, P.O. Box 4500, Oulu, FI-90014 Finland
2Physics Faculty, Saratov State University, Astrachanskaya str., 83, Saratov, 410012, Russia
3Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, 634050, Russia
4ITMO University, Kronverkskiy Prospect 49, St Petersburg 197101, Russia
5Clinic of Neonatology, University Hospital Zurich, Zurich, CH-8091, Switzerland
6Institute of Precision Mechanics and Control, Russian Academy of Sciences, Rabochaya str., 24, Saratov, 410028, Russia
7Institute of Biology, Irkutsk State University, Irkutsk, 66400, Russia
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.8 MB)
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Language: English
Published: IOP Publishing, 2017
Publish Date: 2019-05-02


A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

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Series: Laser physics letters
ISSN: 1612-2011
ISSN-E: 1612-202X
ISSN-L: 1612-2011
Volume: 14
Issue: 10
Article number: 105601
DOI: 10.1088/1612-202X/aa7d38
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
217 Medical engineering
Funding: Authors acknowledge the support provided by CIMO Foundation (CIMO Fellowship, № TM-15-9625), as well as partial support provided by The Ministry of Science and Education of Russian Federation, №3.1340.2014/K., grant project “Development of optical methods and instrumentation for measurements and control of structure and dynamics of biological media”, government contract №2014/203 and by Government of Russian Federation, Grant 074-U01. Professor Meglinski also acknowledges partial support provided by Russian Science Foundation project №15-14-10008.
Copyright information: © 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at