Elina Kylmäoja, Miho Nakamura, Sanna Turunen, Christina Patlaka, Göran Andersson, Petri Lehenkari, Juha Tuukkanen. Peripheral blood monocytes show increased osteoclast differentiation potential compared to bone marrow monocytes. Heliyon 4 (2018) e00780. doi: 10.1016/j.heliyon.2018.e00780
Peripheral blood monocytes show increased osteoclast differentiation potential compared to bone marrow monocytes
|Author:||Kylmäoja, Elina1; Nakamura, Miho1,2; Turunen, Sanna1;|
1Institute of Cancer Research and Translational Medicine, Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, P.O. Box 5000, 90014, Finland
2Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan
3Department of Laboratory Medicine, Division of Pathology F46, Karolinska Institutet and Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden
|Online Access:||PDF Full Text (PDF, 1.7 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2019060518585
|Publish Date:|| 2019-07-04
Bone marrow (BM) and peripheral blood (PB) derived mononuclear cells are precursors of in vitro osteoclast differentiation. However, few studies have compared the phenotypic and functional properties of osteoclasts generated from these sources and the effects of different growth factors on osteoclastogenesis. Both cell types differentiated into functional osteoclasts, but culturing the cells with or without transforming growth factor beta (TGF-β) and dexamethasone revealed differences in their osteoclastogenic capacity. When receptor activator for nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were used for differentiation, we did not observe differences in bone resorption activity or expression of osteoclastogenic genes calcitonin receptor (CR) and nuclear factor of activated T-cells (NFATc1) between the osteoclasts formed from the two sources. Addition of TGF-β and dexamethasone led to higher number of nuclei in multinuclear cells and increased expression of tartrate resistant acid phosphatase (TRACP) 5a and 5b, CR and NFATc1 in PB- derived osteoclasts depicting the higher osteoclastogenic potential and responsiveness to TGF-β and dexamethasone in PB monocytes. These results conclude that the choice of the osteoclast precursor source as well as the choice of osteoclastogenic growth factors are essential matters in determining the phenotypic characteristics of heterogeneous osteoclast populations.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
Miho Nakamura was supported by the Japan Society for Promotion of Science (grant number 15KK0299). Göran Andersson and Christina Patlaka were supported by the Swedish Research Council.
© 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).