University of Oulu

Yanchun Guo, Tatjana Dorn, Susanne J. Kühl, Alexander Linnemann, Melanie Rothe, Astrid S. Pfister, Seppo Vainio, Karl-Ludwig Laugwitz, Alessandra Moretti, Michael Kühl, The Wnt inhibitor Dkk1 is required for maintaining the normal cardiac differentiation program in Xenopus laevis, Developmental Biology, Volume 449, Issue 1, 2019, Pages 1-13, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2019.02.009

The Wnt inhibitor Dkk1 is required for maintaining the normal cardiac differentiation program in Xenopus laevis

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Author: Guo, Yanchun1,2; Dorn, Tatjana3; Kühl, Susanne J.1;
Organizations: 1Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany
2International Graduate School in Molecular Medicine Ulm, Ulm University, 89081 Ulm, Germany
3Klinik und Poliklinik für Innere Medizin I, Klinikum Rechts der Isar der Technischen Universität München, Ismaninger Strasse 22, 81675 Munich, Germany
4Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, InfoTech Oulu, Oulu University and Biobank Borealis of Northern Finland, Oulu University Hospital, Aapistie 5, FIN-90014, University of Oulu, Finland
5DZHK (German Centre for Cardiovascular Research) - Partner Site Munich Heart Alliance, Munich, Germany
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2019061320407
Language: English
Published: Elsevier, 2019
Publish Date: 2020-02-21
Description:

Abstract

Wnt proteins can activate different intracellular signaling pathways. These pathways need to be tightly regulated for proper cardiogenesis. The canonical Wnt/β-catenin inhibitor Dkk1 has been shown to be sufficient to trigger cardiogenesis in gain-of-function experiments performed in multiple model systems. Loss-of-function studies however did not reveal any fundamental function for Dkk1 during cardiogenesis. Using Xenopus laevis as a model we here show for the first time that Dkk1 is required for proper differentiation of cardiomyocytes, whereas specification of cardiomyocytes remains unaffected in absence of Dkk1. This effect is at least in part mediated through regulation of non-canonical Wnt signaling via Wnt11. In line with these observations we also found that Isl1, a critical regulator for specification of the common cardiac progenitor cell (CPC) population, acts upstream of Dkk1.

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Series: Developmental biology
ISSN: 0012-1606
ISSN-E: 1095-564X
ISSN-L: 0012-1606
Volume: 449
Issue: 1
Pages: 1 - 13
DOI: 10.1016/j.ydbio.2019.02.009
OADOI: https://oadoi.org/10.1016/j.ydbio.2019.02.009
Type of Publication: A1 Journal article – refereed
Field of Science: 1184 Genetics, developmental biology, physiology
1182 Biochemistry, cell and molecular biology
Subjects:
Copyright information: © 2019 The Authors. Published by Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/