University of Oulu

Sanna Uusitalo, Martin Kögler, Anna-Liisa Välimaa, Jarno Petäjä, Ville Kontturi, Samuli Siitonen, Riitta Laitinen, Matti Kinnunen, Tapani Viitala, Jussi Hiltunen, “Stability optimization of microbial surfaceenhanced Raman spectroscopy detection with immunomagnetic separation beads,” Opt. Eng. 56(3), 037102 (2017), doi: 10.1117/1.OE.56.3.037102

Stability optimization of microbial surface-enhanced Raman spectroscopy detection with immunomagnetic separation beads

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Author: Uusitalo, Sanna1; Kögler, Martin2,3; Välimaa, Anna-Liisa4;
Organizations: 1VTT Technical Research Centre of Finland, Oulu, Finland
2University of Helsinki, Centre for Drug Research, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, Helsinki, Finland
3Technische Universität Berlin, Chair of Bioprocess Engineering, Institute of Biotechnology, Berlin, Germany
4National Resources Institute Finland (LUKE), Bio-based Business and Industry, Oulu, Finland
5Nanocomp Oy Ltd., Lehmo, Finland
6University of Oulu, Faculty of Information Technology and Electrical Engineering, Optoelectronics and Measurement Techniques Research Unit, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.6 MB)
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Language: English
Published: SPIE, 2017
Publish Date: 2019-06-17


Immunomagnetic separation (IMS) beads with antibody coating are an interesting option for biosensing applications for the identification of biomolecules and biological cells, such as bacteria. The paramagnetic properties of the beads can be utilized with optical sensing by migrating and accumulating the beads and the bound analytes toward the focus depth of the detection system by an external magnetic field. The stability of microbial detection with IMS beads was studied by combining a flexible, inexpensive, and mass producible surface-enhanced Raman spectroscopy (SERS) platform with gold nanoparticle detection and antibody recognition by the IMS beads. Listeria innocua ATCC 33090 was used as a model sample and the effect of the IMS beads on the detected Raman signal was studied. The IMS beads were deposited into a hydrophobic sample well and accumulated toward the detection plane by a neodymium magnet. For the first time, it was shown that the spatial stability of the detection could be improved up to 35% by using IMS bead capture and sample well placing. The effect of a neodymium magnet under the SERS chip improved the temporal detection and significantly reduced the necessary time for sample stabilization for advanced laboratory testing.

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Series: Optical engineering
ISSN: 0091-3286
ISSN-E: 1560-2303
ISSN-L: 0091-3286
Volume: 56
Issue: 3
Article number: 037102
DOI: 10.1117/1.OE.56.3.037102
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Funding: The funding for this project was provided, in part, by TEKES (the Finnish Funding Agency for Technology and Innovation) and University of Oulu Graduate School through Infotech Oulu Doctoral Program. Academy of Finland joined in funding the work through research projects (Foulsens Grant No. 292253 and M-SPEC Grant No. 284907). We thank Tiina Väyrynen (National Resources Institute Finland) for the work with the bacterial sample preparation.
Copyright information: © 2017 SPIE.