University of Oulu

Huemer P, Hebert PDN, Mutanen M, Wieser C, Wiesmair B, Hausmann A, et al. (2018) Large geographic distance versus small DNA barcode divergence: Insights from a comparison of European to South Siberian Lepidoptera. PLoS ONE 13(11): e0206668.

Large geographic distance versus small DNA barcode divergence : insights from a comparison of European to South Siberian Lepidoptera

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Author: Huemer, Peter1; Hebert, Paul D. N.2; Mutanen, Marko3;
Organizations: 1Naturwissenschaftliche Sammlungen, Tiroler Landesmuseen Betriebsges.m.b.H., Innsbruck, Austria
2Centre for Biodiversity Genomics, University of Guelph, Guelph, Canada
3Ecology and Genetics Research Unit, University of Oulu, Oulu, Finland
4Landesmuseum Kärnten, Klagenfurt, Austria
5Section Lepidoptera, Bavarian State Collection of Zoology, Munich, Germany
6Ecology Department, Altai State University, Barnaul, Russia
7Tomsk State University, Tomsk, Russia
8Department of Ecology, University of Innsbruck, Innsbruck, Austria
9Department of Botany & Biodiversity Research, University of Vienna, Vienna, Austria
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.7 MB)
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Language: English
Published: Public Library of Science, 2018
Publish Date: 2019-06-17


Spanning nearly 13,000 km, the Palearctic region provides an opportunity to examine the level of geographic coverage required for a DNA barcode reference library to be effective in identifying species with broad ranges. This study examines barcode divergences between populations of 102 species of Lepidoptera from Europe and South Siberia, sites roughly 6,000 km apart. While three-quarters of these species showed divergence between their Asian and European populations, these divergence values ranged between 0–1%, distinctly less than the distance to the Nearest-Neighbor species in all but a few cases. Our results suggest that further taxonomic studies may be required for 16 species that showed either extremely low interspecific or high intraspecific variation. For example, seven species pairs showed low or no barcode divergence, but four of these cases are likely to reflect taxonomic over-splitting while the others involve species pairs that are either young or show evidence for introgression. Conversely, some of the nine species with deep intraspecific divergence at varied spatial levels may include overlooked species. Although these 16 cases require further investigation, our overall results indicate that barcode reference libraries based on records from one locality can be very effective in identifying specimens across an extensive geographic area.

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Series: PLoS one
ISSN: 1932-6203
ISSN-E: 1932-6203
ISSN-L: 1932-6203
Volume: 13
Issue: 11
Article number: e0206668
DOI: 10.1371/journal.pone.0206668
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
Funding: Promotion of Educational Policies, University and Research Department of the Autonomous Province of Bolzano - South Tyrol, Italy; Austrian Federal Ministry of Science, Research and Economics; Finnish Cultural Foundation; Kone foundation; Academy of Finland; Canada Foundation for Innovation; Ontario Ministry of Research, Innovation and Science; NSERC for their support; Ministry of Education and Science of the Russian Federation,grant No. 6.2884.2017/4.6. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Copyright information: © 2018 Huemer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.