University of Oulu

Väyrynen, O., Åström, P., Nyberg, P., Alahuhta, I., Pirilä, E., Vilen, S., Aikio, M., Heljasvaara, R., Risteli, M., Sutinen, M., Salo, T. (2019) Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells. 376 (1), 18-26. doi:10.1016/j.yexcr.2019.01.018

Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells

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Author: Väyrynen, Otto1,2; Åström, Pirjo1,2; Nyberg, Pia1,2,3;
Organizations: 1Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland
2Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland
3Biobank Borealis of Northern Finland, Oulu University Hospital, Finland
4Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland
5Oulu Center for Cell-Matrix Research and Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Finland
6Centre for Cancer Biomarkers (CCBIO), University of Bergen, Norway
7HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.3 MB)
Persistent link:
Language: English
Published: Elsevier, 2019
Publish Date: 2020-01-30


Pro-tumorigenic activities of matrix metalloproteinase (MMP) 9 have been linked to many cancers, but recently the tumour-suppressing role of MMP9 has also been elucidated. The multifaceted evidence on this subject prompted us to examine the role of MMP9 in the behaviour of oral tongue squamous cell carcinoma (OTSCC) cells. We used gelatinase-specific inhibitor, CTT2, and short hairpin (sh) RNA gene silencing to study the effects of MMP9 on proliferation, motility and invasion of an aggressive OTSCC cell line, HSC-3. We found that the migration and invasion of HSC-3 cells were increased by CTT2 and shRNA silencing of MMP9. Proliferation, in turn, was decreased by MMP9 inhibition. Furthermore, arresten-overexpressing HSC-3 cells expressed increased levels of MMP9, but exhibited decreased motility compared with controls. Interestingly, these cells restored their migratory capabilities by CTT2 inhibition of MMP9. Hence, although higher MMP9 expression could give rise to an increased tumour growth in vivo due to increased proliferation, in some circumstances, it may participate in yet unidentified molecular mechanisms that reduce the cell movement in OTSCC.

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Series: Experimental cell research
ISSN: 0014-4827
ISSN-E: 1090-2422
ISSN-L: 0014-4827
Volume: 376
Issue: 1
Pages: 18 - 26
DOI: 10.1016/j.yexcr.2019.01.018
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
1182 Biochemistry, cell and molecular biology
Funding: This study was financially supported by the Academy of Finland, the Cancer Foundation of Finland, the Finnish Cultural Foundation, the Finnish Dental Society Apollonia, the Cancer Foundation of Northern Finland, the University of Oulu Scholarship Foundation and research funds from the Medical Faculty of the University of Oulu and Oulu University Hospital special state support for research.
Copyright information: © 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license