University of Oulu

Elina Kylmäoja, Miho Nakamura, Hanna Kokkonen-Puuperä, Veli-Pekka Ronkainen, Petri Lehenkari, Juha Tuukkanen. Gap junctional communication is involved in differentiation of osteoclasts from bone marrow and peripheral blood monocytes. Heliyon 4 (2018) e00621.doi: 10.1016/j.heliyon.2018.e00621

Gap junctional communication is involved in differentiation of osteoclasts from bone marrow and peripheral blood monocytes

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Author: Kylmäoja, Elina1; Nakamura, Miho1,2; Kokkonen-Puuperä, Hanna1;
Organizations: 1Institute of Cancer Research and Translational Medicine, Department of Anatomy and Cell Biology, Medical Research Center, P.O. Box 5000, 90014, University of Oulu, Finland
2Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan
3Biocenter Oulu, Light Microscopy Core Facility, P.O. Box 5000, 90014, University of Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.7 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2019070322655
Language: English
Published: Elsevier, 2018
Publish Date: 2019-07-03
Description:

Abstract

Aims: The aim of the study was to compare the influence of gap junctional communication (GJC) in osteoclastogenesis from bone marrow (BM) and peripheral blood (PB) monocytes. These widely used sources differ in purity, since BM cultures contain a significant number of stromal cells. We studied whether stimulation of GJC in BM monocyte/stromal cell cultures differs from the effect in pure PB monocyte cultures. We compared the differentiation also in acidosis, which is a known inducer of bone resorption.

Main methods:: Human BM and PB monocytes were isolated from BM aspirates or whole blood samples. The cells were cultured on human bone slices with osteoclastogenic growth factors and a GJC modulator, antiarrhythmic peptide AAP10, at physiological and acidic pH.

Key findings:: Both BM and PB monocytes differentiated into osteoclasts. Acidosis increased resorption in both cultures but stimulated cell fusion only in BM cultures, which demonstrates the role of stromal cells in osteoclastogenesis. At physiological pH, AAP10 increased the number of multinuclear cells and bone resorption in both BM and PB cultures indicating that GJC is involved in differentiation in both of these osteoclastogenesis assays. Interestingly, in PB cultures at pH 6.5 the stimulation of GJC with AAP10 inhibited both osteoclastogenesis and bone resorption suggesting a different role of GJC in BM and PB monocytes at stressed environment.

Significance: The study is conducted with primary human tissue samples and adds new knowledge on factors affecting osteoclastogenesis from different monocyte sources.

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Series: Heliyon
ISSN: 2405-8440
ISSN-E: 2405-8440
ISSN-L: 2405-8440
Volume: 4
Issue: 5
Article number: e00621
DOI: 10.1016/j.heliyon.2018.e00621
OADOI: https://oadoi.org/10.1016/j.heliyon.2018.e00621
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Funding: This work was supported by the Sigrid Juselius Foundation, Finland, and the Oulu University Scholarship Foundation, Finland.
Copyright information: © 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
  https://creativecommons.org/licenses/by-nc-nd/4.0/